Genes to Cells 表紙ギャラリー

Genes to Cellsオリジナルグッズ



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A puffed-up fugu is wrestling with a toad. The body of the fugu has numerous spots that look like insulin secretory granules and a nucleus-like pattern, just like the pancreatic β-cells. Perhaps triggered by the wrestling, the fugu's body releases insulin. Inspired by a review article in this journal (Izumi (2023). Multiple pathways and independent functional pools in insulin granule exocytosis. Genes Cells 28: 471-481, DOI: 10.1111/gtc.13029).
膨らんだフグとヒキガエルが相撲を取っている。フグの体にはインスリン分泌顆粒のような多数の斑点と核のような模様が見え、まるで膵臓のβ細胞のようだ。そして相撲が契機となったのか、フグの体からはインスリンが放たれている。当誌掲載の総説(Izumi (2023). Multiple pathways and independent functional pools in insulin granule exocytosis. Genes Cells 28: 471-481, DOI: 10.1111/gtc.13029)より着想した。
It has been demonstrated that in Arabidopsis thaliana, the accumulation of the plant hormone jasmonic acid at the base of flowering petals turns on a gene expression cascade there, resulting in the activation of autophagy and vacuolar development, which in turn causes petal shedding (Furuta et al. (2024) Nat. Commun. 15: 1098, DOI: 10.1038/s41467-024-45371-3). Perhaps in the future, we will be able to control the timing of the fall of the petals in other flowering plants, such as cherry blossoms, as we wish.
シロイヌナズナにおいて、開花した花弁の基部へ植物ホルモンであるジャスモン酸が蓄積することで、そこでの遺伝子発現カスケードがオンになり、その結果としてオートファジーの活性化と液胞の発達が起こり、それによって花弁が散ることが示された(Furuta et al. (2024) Nat. Commun. 15: 1098, DOI: 10.1038/s41467-024-45371-3)。将来的には、桜など他の顕花植物でも、散る時期を自在にコントロールできるようになるかもしれない。
Son Goku (Sun Wukong), a god in monkey form, was brandishing a Nyoi-bo representing the TBXT gene. However, an AluY-marked dagger hit the Nyoi-bo and broke it off. Xia et al. showed that the loss of tails in hominoids, including humans and apes, was due in part to changes in splicing patterns caused by the insertion of an AluY element into the ancestral TBXT gene (Xia et al. (2024) Nature 626:1042-1048, DOI: 10.1038/s41586-024-07095-8). Son Goku cannot hide the look of impatience on his face as his tail begins to disappear, having impaired the function of the Nyoi-bo, which is necessary to maintain his tail, the source of Son Goku's magical power.
猿の姿をした神である孫悟空が、TBXT遺伝子をかたどった如意棒を振り回していた。ところが、その如意棒にAluY印の匕首が命中して折れてしまった。Xiaらは、ヒトや類人猿などのヒト上科が尻尾を失った原因の一端は、祖先のTBXT遺伝子にAluY要素が挿入されたことでスプライシングのパターンに変化が生じたことにあることを示した(Xia et al. (2024) Nature 626:1042-1048, DOI: 10.1038/s41586-024-07095-8)。孫悟空の尻尾の維持に必要な如意棒の機能が損なわれたことで、その魔力の源泉である尻尾が消え始めた孫悟空は、焦りの表情を隠しきれない。
This is an ornamental tool called an 'inro' used to carry medicines in the Edo period. On the surface of this inro, the structure of N1-methyl-pseudouridine (N1mΨ), the key to the development of the mRNA vaccine against COVID-19, is schematically depicted with plum buds (oxygen atoms) and branches, using the maki-e lacquer decoration technique. Coronavirus particles are depicted on the toggle ('netsuke') used to secure the inro to the obi of the kimono. Perhaps one day, wherever we travel, we will enjoy our journey without fear of unknown viruses by swallowing a pill taken from our portable pill case like this inro.
A fishmonger skillfully cutting a planarian with a high regenerative capacity creates various forms of planarians, including two-headed and four-headed (the planarians are depicted enlarged).
The year 2024, the Year of the Dragon, was opened. Mt. Fuji was quickly enveloped by colorful clustered single-cell RNA-seq data-like clouds that rose from the dragon targeting the mountain.
A woman observes Danionella in a fishbowl (the fish depicted magnified). Danionella has the advantage of having all its organs transparent throughout its life, making it a promising new model organism for imaging and optogenetics.
Mouse sperm are immature immediately after production in the testis. Lumicrine factors (NELL2 and NICOL) secreted by the testis stimulate the differentiation of epididymal initial segment epithelial cells, and these epithelial cells are responsible for processing the ADAM3 protein on the sperm surface, resulting in sperm maturation. For details, see the review article in this issue (Kiyozumi (2023) Genes Cells 28: 757-763, DOI: 10.1111/gtc.13066). The boys with long hair in this drawing also have their hair cut at the barbershops at the approach to a bridge and go out into the world, crossing the bridge as mature men.
マウスの精子は、精巣で作られた直後は未成熟である。精巣から分泌されたルミクリン因子(NELL2とNICOL)が精巣上体頭部の上皮細胞の分化を促し、その上皮細胞の働きで精子表面のADAM3タンパク質がプロセシングを受け、精子は成熟する。詳しくは今月号に掲載の総説を参照(Kiyozumi (2023) Genes Cells 28: 757-763, DOI: 10.1111/gtc.13066)。この絵の長い髪の男の子たちも、橋のたもとの床屋で髪を切り、成熟した男となって橋を渡り、外の世界へ出発していくのである。
An awake mouse is pulling to move a soundly sleeping mouse by the tail. The tail of the awake mouse is coiled just like the 3D structure of orexin-A, a hormone that regulates arousal.
分裂酵母国際会議 in 広島
A full moon rises over the torii gate of Itsukushima Shrine in Miyajima (Hiroshima Prefecture), with countless reflections on the waves. Upon closer inspection, the numerous moon figures seen on the waves are actually cells of the fission yeast Schizosaccharomyces pombe. In addition to cells undergoing vegetative growth, cells in the process of sexual reproduction (conjugation, first and second meiosis) and asci containing spores are visible in the foreground. The Meeting Report of the 11th International Fission Yeast Meeting in Hiroshima (POMBE2023 Hiroshima) appears in this month's issue of this journal (Toda et al., Genes Cells 28: 642-645, DOI: 10.1111/gtc.13055).
宮島(広島県)の厳島神社の鳥居を背景に満月が昇り、波間にその姿が無数に反射している。よく見ると、波間に見える月の姿の正体は分裂酵母Schizosaccharomyces pombeの細胞だった。栄養増殖中の細胞に加え、手前側には有性生殖過程(接合、第一・第二減数分裂)にある細胞と、胞子を含んだ胞子嚢も見える。本誌今月号に、広島で開催された第11回国際分裂酵母会議(POMBE2023 Hiroshima)のミーティングレポートが掲載されている(Toda et al., Genes Cells 28: 642-645, DOI: 10.1111/gtc.13055)。
Morning glories bloom on an August morning. The pattern of the flowers is reminiscent of paraspeckles, which are nonmembranous organelles formed by lncRNA Neat1. Also, the tendril extending right-upwards looks like Hero proteins, which are intrinsically disordered. A review article on nondomain biopolymers, including Neat1 and Hero proteins, appears in this issue of this journal (Arakawa et al. (2023) Genes Cells 28: 539-552, DOI: 10.1111/gtc.13050).
8月の朝に咲き誇るアサガオ。その花の模様はlncRNA Neat1によって形成される非膜性オルガネラであるパラスペックルを彷彿とさせる。また、右上方へ伸びる蔓は、まるで天然変性タンパク質であるHeroタンパク質のようだ。本誌今月号に、Neat1やHeroタンパク質のような非ドメイン型バイオポリマーに関する総説が掲載されている(Arakawa et al. (2023) Genes Cells 28: 539-552, DOI: 10.1111/gtc.13050)。
Finally, today is the deadline for abstract submission for the annual meeting of the Molecular Biology Society of Japan. As the deadline is approaching, the front of the building, where the registration desk is situated, is getting crowded with people having their abstracts to submit. In the next building, a few people are in a hurry as they have not yet completed their abstracts, and the woman at the far end of the cover illustration of the June issue is there too. The abstract submission deadline for this year's annual meeting (MBSJ2023) is July 31, 17:00 (JST). Your early submission is highly appreciated.
The abstract registration deadline for the annual meeting of the Molecular Biology Society of Japan on July 31 is 40 days ahead. Here is a scene in a laboratory. As is usual, the preparation of abstracts has begun. The degree of progress varies from person to person; some have already received corrections from the faculty members, while others, like the person at the far end of the room, are taking a slower pace. What will the deadline they will face be like!? (To be continued in the next issue; for a hint, see the meeting report on MBSJ2022 in this issue (Fukagawa (2023) Genes Cells 28: 403-410, DOI: 10.1111/gtc.13023).)
7月31日の日本分子生物学会年会の要旨登録締切まで40日となった、とある研究室。ここでも要旨の準備が始まっている。進行の度合いは人により様々で、すでに教員による添削を受けている者もいれば、最も奥の人物のように遅めの者もいる。はたして彼らはどのような締切日を迎えるのだろう!?(来月号へ続く;ヒントは今号掲載のMBSJ2022のミーティングレポート(Fukagawa (2023) Genes Cells 28: 403-410, DOI: 10.1111/gtc.13023)をご覧ください。)
Stimulated to the leaves, the sensitive plant (Mimosa pudica) folds its leaves one after another. To elucidate the mechanism, a transgenic M. pudica was produced so that the cytosolic Ca2+ concentration can be observed as fluorescence by GCaMP6f (Hagihara et al. (2022) Nat. Commun. 13: 6412, DOI: 10.1038/s41467-022-34106-x). When a leaflet was wounded, the fluorescent signal caused by Ca2+ propagated through the veins to the tertiary pulvinus at the base of the leaflet, immediately followed by a movement of the pair of leaflets to fold inwards. And, Ca2+ signals were propagated to the adjacent tertiary pulvini sequentially, and the leaflets were also folded successively. At this time, electrical signals were transmitted simultaneously with the fluorescent signals. The authors also observed that the immotile M. pudica was more susceptible to feeding damage by insects, suggesting that the movement above protects the plant from feeding damage.
葉へ刺激を受けたオジギソウ(Mimosa pudica)は次々に葉を折りたたむ。その機構を明らかにするため、細胞質のCa2+濃度をGCaMP6fにより蛍光として観察できる遺伝子組換えオジギソウが作製された(Hagihara et al. (2022) Nat. Commun. 13: 6412, DOI: 10.1038/s41467-022-34106-x)。小葉を傷つけると、葉脈を通じてその小葉の付け根の小葉枕へとCa2+による蛍光シグナルが伝播し、直後に小葉の対を折りたたむ運動が起こった。そして隣接する小葉枕へも次々とCa2+シグナルが伝播し、小葉も続々と折りたたまれていった。さらに、蛍光シグナルと同時に電気シグナルも伝わっていた。著者らは、動かないオジギソウは昆虫に食べられやすいことも観察しており、この動きは植物を食害から守っていると考察している。
Wnt2022, an international conference on Wnt research, was held on November 15 to 19, 2022, in Awaji Island (Hyogo Prefecture). This memorable gathering was the first Wnt meeting in Japan and Asia, finally held in person after being postponed for two years due to COVID-19. The cover of this issue features Himeji Castle (Himeji, Hyogo Prefecture) (*CG fiction) with an exceptional decoration showing the name of the meeting and the names of important components of Wnt signaling, to commemorate this occasion. The Meeting Report in this issue detailed the discussion at the conference and its connection to Himeji Castle (Kikuchi et al. (2023) Genes Cells, 28: 249-257, DOI: 10.1111/gtc.13014).
Wnt研究の国際会議であるWnt2022が、2022年11月15日~19日に淡路島(兵庫県)で開催された。日本そしてアジアでのWntミーティング開催は初めてであったことに加え、COVID-19による2年の延期を経てついに対面形式での開催が叶ったという記念すべき会となった。今月号の表紙は、その開催を記念して、この会議名やWnt シグナリングの重要な構成因子名を表した特別な装飾が施された姫路城(兵庫県姫路市)(※CGによるフィクション)である。会議での議論や姫路城との関連は、今月号掲載に掲載されているミーティングレポート(Kikuchi et al. (2023) Genes Cells, 28: 249-257, DOI: 10.1111/gtc.13014)で詳報されている。
The node forming transiently in mammalian embryos plays a key role in left-right axis determination. The node is a cavity, in which crown cells with immotile cilia surround pit cells that rotate their motile cilia to generate a leftward fluid flow (nodal flow). Experiments using GCaMP6, which emits green fluorescence in response to Ca2+, and optical tweezers revealed that immotile cilia sense mechanical force generated by nodal flow (Katoh et al. (2023) Science 379: 66-71, DOI: 10.1126/science.abq8148). A similar mechanism was shown to exist in zebrafish (Djenoune et al. (2023) Science 379: 71-78, DOI: 10.1126/science.abq7317). This picture shows the application of this mechanism to 'hiburi-ryo' (fishing by using flames to drive fish). The fishermen in the pond are creating a leftward current to be sensed by the men on the left bank, who are activated, and their torch flames intensify.
哺乳類の胚に一時的に形成されるノードは左右軸決定に重要な役割を果たす。ノードはくぼんだ形をしており、繊毛を回転させて左向きの水流(ノード流)を発生させるピット細胞の周囲を、不動繊毛を持つクラウン細胞が取り囲んでいる。Ca2+に反応して緑色の蛍光を発するGCaMP6と光ピンセットを用いた実験により、クラウン細胞の不動繊毛がノード流により生み出された機械的な力を感知していることが明らかになった(Katoh et al. (2023) Science 379: 66-71, DOI: 10.1126/science.abq8148)。ゼブラフィッシュでも同様の機構が存在することが示された(Djenoune et al. (2023) Science 379: 71-78, DOI: 10.1126/science.abq7317)。この画はその仕組みを火振り漁(炎で魚を追い込む漁)に応用している様子である。池の中の漁師たちは左向きの流れを作り出し、それを感じ取った左岸の男達が活性化され、彼らの松明の炎も強まっている。
Urashima Taro, the protagonist of a Japanese fairy tale, returned from his undersea utopia with his youth intact after hundreds of years, but as soon as he was exposed to the smoke from the souvenir Tamatebako (jeweled box), he turned into an old man. This painting depicts how the smoke causes Urashima Taro to lose not only his youth but also the Y chromosome in a part of his cells. Mosaic loss of chromosome Y (mLOY) is commonly observed in older men and has been linked to ageing and cancer, and has also been found to increase the risk of heart disease (Sano et al. (2022) Science 377: 292-297, DOI: 10.1126/science.abn3100). We referred to Fukami & Miyado (2022) Reprod. Med. Biol. 21: e12445, DOI: 10.1002/rmb2.12445 for the depiction of chromosomes.
浦島太郎は日本のお伽話の主人公であり、海底にある理想郷から数百年ぶりに若さを保ったままで戻ったが、土産の玉手箱から出た煙を浴びた途端に老人の姿となったという。この画は、その煙が浦島太郎から若さだけでなく一部の細胞のY染色体をも失わせる様子を描いている。Y染色体のモザイク喪失(mosaic loss of chromosome Y (mLOY))は高齢男性でよく観察され、老化やがんとの関係が指摘されているほか、心疾患のリスクを高める(Sano et al. (2022) Science 377: 292-297, DOI: 10.1126/science.abn3100)ことも明らかとなった。染色体の描写はFukami & Miyado (2022) Reprod. Med. Biol. 21: e12445, DOI: 10.1002/rmb2.12445 を参考にした。
Fukuwarai is a traditional Japanese New Year's game in which blindfolded players compete on how well or strangely they can create faces by placing cut-out facial parts (eyes, nose, etc.) on paper with an outline of a face. What these women are enjoying is a fukuwarai with cell organelles likened to facial parts. Now, what kind of cells will they make?
Two pairs of “look-alikes” that match SNPs. Comparisons of multi-omics and lifestyle were made between look-alikes who were not genetically related but had similar faces (Joshi et al. (2022) Cell Reports 40: 111257, DOI: 10.1016/j.celrep.2022.111257). The results showed that among pairs of “look-alike,” the rate of matching SNPs related to facial features was high. In addition, height, weight, smoking habits, and education also had correlations. On the other hand, DNA methylome and microbiome were not similar. This study may provide clues as to how much the extent to which the genome sequence and environmental factors contribute to physical and behavioral traits.
SNPが一致している2組の「そっくりさん」。血縁関係はないが顔が似ているそっくりさん同士でマルチオミックスと生活習慣の比較が行われた(Joshi et al. (2022) Cell Reports 40: 111257, DOI: 10.1016/j.celrep.2022.111257)。その結果、そっくりさんペア内では顔貌に関係するSNPが一致する率が高く、加えて身長・体重・喫煙習慣・教育歴も相関していることが分かった。一方でDNAメチロームやマイクロバイオームは似ていなかった。この研究は、ゲノム配列と環境要因が身体的・行動的特徴にどの程度寄与するのかという問いへのヒントになるかもしれない。
People are fleeing from a tornado that has suddenly appeared. The tornado swallows a bundle of rope with shide (paper ornaments attached to ropes at Japanese shrines) as it unravels and tears the rope into small pieces to spit them out, just like the 26S proteasome that recognizes and degrades ubiquitinated target proteins. Inspired by
突如出現した竜巻に逃げ惑う人々。竜巻は、紙垂(しで)(日本の神社で縄に付けられる紙の飾り)の付いた縄の束を解きながら飲み込んでいき、小片に引き裂いて吐き出していく。まるでユビキチン化された標的タンパク質を認識して分解する26Sプロテアソームのように。 から着想を得た。
A frog and a lizard rush ahead in the race to live out their lifespan, while a turtle walks slowly at its own pace. Based on a large amount of data from long-term observations of the aging and longevity of various tetrapod ectotherms in the wild, Reinke et al. attributed the longevity of turtles to the physical protection of their bony shells and their relatively slow pace of life (Reinke et al. (2022) Science, 376: 1459-1466, DOI: 10.1126/science.abm0151). The evolutionary process of turtles never ceases to fascinate us. For drawing the phylogenetic tree, we referred to the genome decoding ( by Dr. Shigeru Kuratani (RIKEN) et al.
天寿を全うするというレースで先を急ぐカエル・トカゲと、自らのペースでゆっくり歩むカメ。Reinkeらは、野生のさまざまな四足外温動物の老化と寿命を長期にわたって観察した膨大なデータを基に、カメが長寿である理由は、骨質の甲羅という物理的保護の存在と、カメの比較的ゆっくりとした生活ペースにあると考察した(Reinke et al. (2022) Science, 376: 1459-1466, DOI: 10.1126/science.abm0151)。カメの進化の過程は我々を魅了して止まない。系統樹は倉谷滋博士(理研)らのゲノム解読(を参考に描画した。
A general-purpose humanoid karakuri ningyo (puppet) robot that accurately dispenses reagents. Saving labor is not the only thing such general-purpose robots for laboratories can do. The reproducibility of research could be dramatically improved by quantifying human skills and experience and setting them to robots. It will also facilitate scaling up of research. Sharing of programs (protocols) could make it easier for everyone to try highly challenging experiments.
Sugashima Island in Toba City, Mie Prefecture, was depicted in Hiroshige Utagawa's ukiyoe (below 'Cells' in this painting). A report on yeasts and filamentous fungi collected around the Sugashima Marine Laboratory, Graduate School of Science, Nagoya University (NU-MBL) on this island has been published in the February 2022 issue of this journal (Goshima (2022) Genes to Cells 27: 124-137, DOI: 10.1111/gtc.12916). It was the first article in this journal to explore new marine microorganism species. In this article, the author also observed that three species of marine black yeasts changed their mode of growth and division in response to cell density. We are reminded once again that the seas are a treasure trove of biodiversity. We thank Prof. Gohta Goshima (Director, NU-MBL) for providing the data.
歌川広重の浮世絵にも描かれた三重県鳥羽市の菅島(画の中の 'Cells' の直下に描かれている)。ここにある名古屋大学大学院理学研究科附属菅島臨海実験所(NU-MBL)周辺で採取された酵母と糸状菌に関する報告が、当誌の2022年2月号に掲載されている(Goshima (2022) Genes to Cells 27: 124-137, DOI: 10.1111/gtc.12916)。新種の海生微生物を探索した論文は、当誌では初めてである。この論文で著者は、3種の海生黒色酵母が細胞の密度に応じて生長と分裂の様式を変えることも観察している。海が生物多様性の宝庫であることに改めて気づかされる。データを提供頂いた五島剛太教授(NU-MBL所長)に感謝申し上げる。
Dyed cloths are hung out to dry and flutter coolly. Those cloths represent human 22 autosomes and X chromosome. Although it has been a long time since most of the human genome was sequenced, there were still many unresolved parts (the colored parts of the cloths in this drawing), such as telomere, centromere, ribosomal DNA, and segmental duplications. Thanks to the efforts of researchers and advances in technology, the sequencing of all bases except the Y chromosome has been finally achieved (Nurk et al. (2022) Science 376: 44-53, DOI: 10.1126/science.abj6987).
染め上げられた布が干され、涼しげにはためいている。それらの布は、ヒトの22本の常染色体とX染色体を表している。ヒトゲノムの大部分の解読が報告されて久しいが、テロメア・セントロメア・リボソームDNA ・セグメント重複など、未解読の部分(図中の布の着色部分)が多数残されていた。研究者たちの努力と技術の進歩により、Y染色体を除いた全塩基の解読がついに達成された(Nurk et al. (2022) Science 376: 44-53, DOI: 10.1126/science.abj6987)。
Barbour et al. created an experimental food web, as depicted in this drawing, consisting of four species, Arabidopsis thaliana, two species of aphids, and a parasitoid wasp parasitizing the aphids, and examined the effects of alleles of three genes in the aliphatic glucosinolate biosynthesis pathway in A. thaliana on the survival of the four species (Barbour et al. (2022) Science 376: 70-73, DOI: 10.1126/science.abf2232). Their finding was that the null allele of one gene (AOP2) significantly reduced the extinction rate of the four. They call AOP2 a 'keystone gene,' in analogy to a keystone species. We would like to thank Dr. Toshiyuki Harumoto (Hakubi Center for Advanced Research, Kyoto University) for his advice on the drawing.
Barbourらは、この絵のようなシロイヌナズナ・2種類のアブラムシ・アブラムシに寄生する寄生バチの4種からなる実験的な食物網をつくり、シロイヌナズナの脂肪族グルコシノレート生合成経路の3種類の遺伝子のアレルが4種の生存に与える影響を調べた(Barbour et al. (2022) Science 376: 70-73, DOI: 10.1126/science.abf2232)。そして彼らは、ある1つの遺伝子(AOP2)の欠失アレルにより、4種の絶滅率が著しく低下することを発見した。彼らは、キーストーン種になぞらえて、AOP2のことを「キーストーン遺伝子」と呼んでいる。作画への助言をいただいた春本敏之博士(京都大学白眉センター)に感謝申し上げる。
Numerous rafts and small boats float in the river, which flows in the shape of a cup, surrounding a pond with an islet. They look as if they are part of the cells of the green alga Chlamydomonas. In the chloroplasts surrounding the nucleus, thylakoids overlap each other to form the grana. The long rafts in particular look like lamellae interconnecting the grana. The central sandbar with the rafts moored to it resemble a pyrenoid (a structure found in the chloroplasts of algae) with thylakoid membranes penetrating the pyrenoid itself. We referred to Figure 1(A) of Engel et al. (2015) eLife 4:e04889, DOI: 10.7554/eLife.04889.001. We would like to thank Dr. Takashi Yamano (Graduate School of Biostudies, Kyoto University) for his advice.
小島のある池を取り囲むように、カップの形に流れる川に、筏と小舟が多数浮かんでいる。その様子はまるで、緑藻クラミドモナスの細胞内の一部分のように見える。核を取り囲むように位置する葉緑体の中で、チラコイドが重なり合ってグラナを形成している。特に長い筏はグラナどうしを繋ぐラメラのようだ。そして中央にある中州と、それに筏が係留されているさまは、ピレノイド(藻類の葉緑体に見られる構造)と、それに貫入しているチラコイド膜の様子に似ている。Engel et al. (2015) eLife 4:e04889, DOI: 10.7554/eLife.04889.001のFigure 1(A)を参考にした。助言をいただいた山野隆志博士(京都大学大学院生命科学研究科)に感謝申し上げる。
One Japanese bullfinch (Pyrrhula pyrrhula griseiventris), which likes to eat cherry blossoms, perches on a drooping cherry branch. This cherry tree has particularly well-shaped branches, and if we compare its flowers to five- or six-membered rings and the buds to sulfur atoms, the form of the flowers, buds and branches around the bird resemble the structure of methionine (left) and its metabolites, S-adenosylmethionine (SAM; top) and S-adenosylhomocysteine (SAH; right). It has been reported that restricting methionine intake extends lifespan in a variety of species. Hopefully this bullfinch doesn't eat too many flowers.
桜の花を好んで食べる鷽(ウソ)(Pyrrhula pyrrhula griseiventris)が一羽、しだれ桜の枝に止まっている。この桜は特に枝の形がよく、花を五員環あるいは六員環に、つぼみを硫黄原子にそれぞれ見立てると、鳥の周りの花、つぼみと枝の形がメチオニン(左)とその代謝物であるS-アデノシルメチオニン(SAM; 上)、S-アデノシルホモシステイン(SAH; 右)の構造に似ている。さまざまな生物種で、メチオニン摂取を制限すると寿命が延長するという報告がある。この鷽も花を食べ過ぎなければよいのだが。
The Hi-C method (a method to examine genomic regions that are close to each other in the nucleus by fragmenting and ligating cross-linked nuclear chromatin and then reading the paired-end sequences by NGS) has revealed the existence of structural units called topologically associating domains (TADs) that are separated by insulators in the genome. When the results of the Hi-C method are drawn as a heatmap, TADs appear as characteristic diamond-shaped signals, with deeper colors indicating the higher probability that regions of the genome are closer to each other. We drew them on Hishimochi rice cakes (diamond-shaped rice cakes), an essential decoration for the Japanese Doll's Festival on March 3. We thank Drs. Ichiro Hiratani and Hisashi Miura (RIKEN BDR) for providing the data.
Hi-C法(クロスリンクした核クロマチンを断片化・リガーゼ処理し、NGSでペアエンドの塩基配列を読むことで、核内で互いに近接しているゲノム領域を調べる方法)により、ゲノムにはインシュレーターにより隔てられたtopologically associating domain(TAD)という構造単位が存在することが明らかとなってきた。Hi-C法で得た結果を、ゲノム上の領域どうしが互いに近接している確率が高いほど色が濃くなるヒートマップとして描くと、TADが特徴的なダイヤモンド型のシグナルとして浮かび上がる。それを、日本の3月3日の桃の節句の飾り付けに欠かせない菱餅の上に描いてみた。データを提供していただいた平谷伊智朗博士と三浦尚博士(理研BDR)に感謝申し上げる。
When translation by the ribosome is stalled, the subsequent ribosome collides with the stalled ribosome, resulting in the accumulation of abnormal proteins. To prevent this occurring, the cell recognizes the specific structures caused by the ribosome collisions, and marks the stalled ribosome for removal. In budding yeast, collisions are detected by the E3 ubiquitin ligase Hel2, and the uS10 of the 40S subunit of the stalled ribosome is marked with a K63-branched ubiquitin chain. The two ribosome-like Yukidarumas (snowmen) in this picture also collide, and the male watcher is marking the one to be removed with tags that mimic a ubiquitin chain. For drawing this image, we referred to a website
At a New Year’s banquet, an entertainer is performing a juggling trick in which a DNA-like string is temporarily cleaved and another string passes through it using a eukaryotic topoisomerase II-mimicking ‘top.’ This trick requires ATP. In humans, topoisomerase IIα is highly expressed in proliferating cells and is essential for DNA replication, transcription, and structural maintenance and proper segregation of mitotic chromosomes, resulting in an important target for anticancer drugs.
正月の宴席で真核生物トポイソメラーゼIIを模した独楽(英語では ‘top’)で、DNA様の紐が一時的に切断され、その間を別の紐が通り抜けるという曲芸を披露する曲芸師。この技にはATPを必要とする。ヒトではトポイソメラーゼIIαが増殖中の細胞で多く発現しており、DNA複製・転写・M期染色体の構造の維持と正常な分配に必須であることから、抗がん剤のターゲットとして重要となっている。
This year's Annual Meeting of the Molecular Biology Society of Japan (MBSJ2021) is going to be held in Yokohama from December 1 to 3. This is the tenth Annual Meeting in Yokohama, but this will be the last. Online participation is also possible if preferred. We would like to express our sincere respect to all the people involved in the preparation of the event despite the difficulties in the COVID-19 pandemic. The participants are arriving at the train station one after another. Let's meet in Yokohama (or online)!
A woman enduring the heat of moxibustion, using a box with chili peppers on top as a pillow. The nonselective cation channel TRPV1 (schematically depicted on her kimono and the side of the box below her) is a receptor that responds to capsaicin, the pungent component of chili pepper, and heat above 43°C, making us feel heat and pain. Prof. David Julius, who discovered TRPV1, will be awarded the Nobel Prize in Physiology or Medicine in 2021 (jointly with Prof. Ardem Patapoutian for the discovery of receptors for mechanical stimuli).
A competition of protein 3D structure prediction. It is about how similar the predictions are to the correct answer (the actual experimental result), which is slightly to the right of the center. The prediction placed on the left of the correct answer was done by an AI, and won the first prize. AI has made remarkable progress in recent years, and in a contest held in 2020 (CASP14), the predictions made by AlphaFold 2, an AI developed by DeepMind, finally reached a level comparable to actual experimental results. The 3D structures shown here were drawn with PyMOL based on the PDB files of target ID T1053 and its prediction models downloaded from CASP14 website (
タンパク質立体構造予測の結果を集めた品評会。中央少し右寄りにある正解(実際の実験結果)にどれだけ近いかが争われる。その左隣のAIによる予測が第1位を獲得した。近年のAIの進歩はめざましく、2020年に開催されたコンテスト(CASP14)では、DeepMindが開発したAIであるAlphaFold 2による予測がついに実際の実験結果に匹敵するレベルに達した。ここに挙げた立体構造は、CASP14のウェブサイト(よりダウンロードしたターゲットID T1053とその予測モデルのPDBファイルを基にPyMOLで描画した。
不可能を可能に 著しく進歩する光遺伝学
Advances in optogenetic techniques, in which light-activated proteins that respond to specific wavelengths are expressed in specific cells, have made it possible to manipulate the activity of specific nerves in many animal species including mammals with high temporal and spatial precision. The method of using optical fiber for light irradiation (right) is highly invasive and restricts behavior of animal. However, new methods to overcome this problem have emerged. Some examples are a method of using implantable wireless light-emitting devices (top left), and a method of injecting upconversion nanoparticles that emit visible light in response to near-infrared (NIR) lights that reach deep into the body (center; the light emitted from Uchide-no-kozuchi (magic mallet) of Daikokuten is NIR).
Kikkoden is an event that used to be held on July 7th of the old lunisolar calendar, and is the prototype of Tanabata (Star Festival). On that day, people offered fruits and threads in hopes of improving their handicraft skills. We will follow the same practice and offer serial and coronal sections of the brain and watermelon rinds sliced in the hope of improving our skill of making paraffin-embedded serial sections with a microtome. In drawing the serial and coronal sections of the brain, we referred to G. Paxinos and C. Watson (1998) The Rat Brain in Stereotaxic Coordinates, 4th ed., Academic Press.
乞巧奠(きっこうでん)は旧暦7月7日に行われていた行事で、七夕(星祭り)の原型である。その日に、人々は手芸技術の向上を願って果物や糸を供えた。我々もそれに倣い、ミクロトームによるパラフィン包埋連続切片作成の技術向上を願って、脳の連続切片と、スイカの皮をスライスしたものを供え物とする。脳の切片を描くにあたって、G. Paxinos and C. Watson (1998) The Rat Brain in Stereotaxic Coordinates, 4th ed., Academic Pressを参考にした。
The night of Tanabata (Star Festival) on July 7th. A heat map of metabolic reactions in each tissue of C. elegans, derived from single-cell transcriptome analysis, illuminates the surface of the sea. And the metabolic networks of each tissue drawn based on the heat map decorate the night sky like constellations floating in the Milky Way. For drawing this image, we referred to Yilmaz et al. (2020) Mol. Syst. Biol. 16:e9649, DOI: 10.15252/msb.20209649.
7月7日の七夕(星祭り)の夜。単一細胞トランスクリプトーム解析から得られた線虫の組織ごとの代謝反応のヒートマップが海面を照らす。そして、それを基にして描かれた組織ごとの代謝ネットワークが、まるで天の川に浮かぶ星座のように夜空を彩った。Yilmaz et al. (2020) Mol. Syst. Biol. 16:e9649, DOI: 10.15252/msb.20209649 を参考にした。
The heat map: Adapted from Figure 3(C) of © Yilmaz et al. (2020) Mol. Syst. Biol. 16:e9649, DOI: 10.15252/msb.20209649. Licensed under CC BY 4.0.
Even in the same mammal, the time it takes for development is very different between humans and mice. The period of the segmentation clock in humans is about 5 hours, whereas that in mice is about 2 hours. The key regulator of the segmentation clock is the transcription factor HES7, whose expression level periodically increases or decreases by self-repression. Experiments in which human and mouse HES7 loci were swapped showed that differences in the speeds of intracellular biochemical reactions, such as gene expression and protein degradation, between species, rather than the sequences of HES7 loci themselves, produce the period difference. This finding suggests that the period difference of the segmentation clock may also be related to the difference in time scales in development between elephants and mice. We thank Dr. Miki Ebisuya (EMBL Barcelona) for her advice.
同じ哺乳類でも、ヒトとネズミでは発生にかかる時間は大きく異なる。ヒトの体節時計の周期は約5時間なのに対して、ネズミでは約2時間である。体節時計のキーレギュレーターは、自己抑制により発現量が周期的に増減する転写因子HES7である。ヒトとネズミのHES7座位を交換する実験により、HES7座位の配列そのものではなく、遺伝子発現やタンパク質分解などの細胞内の生化学的反応速度の種間での違いが、周期の違いを生み出していることが示された。この発見は、体節時計の周期の差が、ゾウとネズミの間での発生における時間スケールの差にも関係する可能性を示唆している。助言をいただいた戎家美紀博士(EMBL Barcelona)に感謝申し上げる。
This “Daruma Otoshi” game imitates the VDJ recombination of the T cell receptor β (TCRβ) locus. In the first step, the DNA sequence between one D and one J is removed, and in the second step, the DNA sequence between one V and the said D is removed. The series of reactions is catalyzed by RAG1/RAG2. The current situation is that the first stage has been successfully completed and we are in the middle of the second step, that is, the DNA sequence between Vβ3-1 and Dβ1 is being knocked out with a hammer marked with RAG1 and RAG2. Since there are two alleles, there are two chances, but if both fail, the cell will undergo apoptosis.
このだるま落としはT細胞受容体β(T-cell receptor β; TCRβ)遺伝子座のVDJ組換えをかたどっている。第一段階では一つのDと一つのJの間のDNA配列が取り除かれ、第二段階では一つのVとそのDとの間のDNA配列が除去される。一連の反応はRAG1/RAG2に触媒される。現在の状況は、第一段階が無事に済み、第二段階の最中、つまりVβ3-1とDβ1の間のDNA配列がRAG1とRAG2の印のついたハンマーで叩き出されているところである。アリルが2本あるのでチャンスは2回あるが、2回とも失敗すると細胞はアポトーシスを起こしてしまう。
Several laser beams shot from heaven onto the mountain, and a part of the mountain body was scattered. It is just like a laser ionization of a mixture of matrix and sample in a mass spectrometer using the MALDI (Matrix Assisted Laser Desorption/Ionization).
天から山にいく筋ものレーザー光が照射され、山体の一部が飛び散った。ちょうどそれは、MALDI法(Matrix Assisted Laser Desorption/Ionization; マトリックス支援レーザー脱離イオン化法)による質量分析計においてマトリックスと試料の混合物がレーザーでイオン化される様子に似ている。
A woman washing two woven cloths in the river. The cloths are intertwined with each other like a double helix of DNA. What she is using to unwind them is a ring-shaped tool that imitates the hexamer of MCM (minichromosome maintenance) 2-7, a DNA helicase.
織り上げた二筋の布を川で洗う女性。布はまるでDNAの二重らせんのように互いに絡み合っている。これを解消するのに女性が使っているのは、DNAヘリカーゼであるMCM(minichromosome maintenance)2-7の六量体を模したリング状の道具である。
As with brown adipocytes, beige adipocytes are rich in mitochondria expressing Uncoupling Protein 1 (UCP1) and produce heat. However, their origin is white adipocytes, and their differentiation is induced by exposure to cold environments or stimulation with certain antidiabetic drugs. Now, in the cold snow scene where the induction of beige adipocytes is likely to progress, the snowball made by the children seems to have been changing into a beige adipocyte-like ball perhaps due to some stimulus.
ベージュ脂肪細胞は褐色脂肪細胞と同様に脱共役タンパク質UCP1(Uncoupling protein 1)を発現したミトコンドリアに富み、熱産生を行う。しかしその由来は白色脂肪細胞であり、寒冷環境への曝露やある種の糖尿病治療薬により分化が誘導される。今、ベージュ脂肪細胞の誘導が進みそうな寒い雪景色の中、子ども達が作った雪玉に、何か刺激が加わったのか、ベージュ脂肪細胞様に変化してきたようだ。
The first sun of the year is rising above the ocean. Several lines of shadows that are emerging between the waves look like a ladder of a multicolored molecular weight marker for SDS-PAGE. It is very auspicious that they appear to be running horizontally and straight without smiling. We hope that this year will be a happy one for you all.
Many academic societies, including MBSJ, hold their annual meetings online this year. This woman is one of the participants, taking part in an online session via a tablet device behind the bamboo blinds. Guessing from her headset with microphone, she might be one of the presenters.
The involvement of liquid droplets formed by liquid-liquid phase separation (LLPS) in various life phenomena has attracted attention. What is depicted in this picture is the Naruto Strait between Shikoku and Awaji Island. In this strait, extremely strong tidal currents occur several times a day, forming huge whirlpools. Now, triggered by the change in the "phase" of the ocean current, enormous liquid droplets formed by LLPS have appeared in the strait along with the whirlpools.
Two drawings in which Hotei dolls with sumo umpire's fans (gunbai) are lined up. The dolls, the gunbais, and the difference of colors of the dolls' clothes represent cells, cadherins, and the difference of subtypes of cadherins, respectively. The drawing on the left depicts the state in which cadherin haven't been concentrated anywhere on the cell membrane yet, and it is reflected in the random arrangement of the dolls. On the other hand, in the drawing on the right, cadherins are concentrated at intercellular junctions, those of the same subtype are bound to each other, and as a result, dolls expressing the same subtype of cadherin are next to each other.
Goldfish (Carassius auratus) with the telescope-eye, dorsal-fin-loss or long-tail phenotype are swimming calmly in a large fishbowl followed by chromosomes. Goldfish experienced a whole-genome duplication 8-14 million years ago, making it difficult to analyze its genome, but finally the genome sequences of various varieties were sequenced and the causative genes (candidates) of these traits were also found. Among these, the causative gene (candidate) for long-tail, kcnk5bS, encodes a K+ channel as depicted on the side of the bowl. This research is expected to be applicable to the study of vertebrate morphogenesis and human disorders. See Chen et al. (2019) Science Advances 5: eaav0547 and Kon et al. (2020) Current Biology 30: 2260-2274 for further detail. We thank Prof. Yoshihiro Omori (Nagahama Institute of Bio-science and Technology) for his advice on drawing.
大きな金魚鉢の中を、染色体を従えて悠然と泳ぐ、出目・背びれなし・長い尾ひれの表現型のキンギョ(Carassius auratus)。キンギョは800~1400万年前に全ゲノム重複を起こしており、ゲノムの解析が困難であったが、ついにさまざまな品種のゲノム配列が解読され、これらの形質の原因遺伝子(候補)が見つかった。この中で、長い尾ひれの原因遺伝子(候補)kcnk5bSは、鉢の側面に描かれているようなK+チャネルをコードしている。この研究は脊椎動物の形態形成やヒトの疾患の研究へ応用できると期待される。詳しくはChen et al. (2019) Science Advances 5: eaav0547とKon et al. (2020) Current Biology 30: 2260-2274を参照。作画に助言をいただいた大森義裕教授(長浜バイオ大学大学院)に感謝申し上げる。
A scene of Yoiyama (the eve of 'Yamahoko Junko' (decorated floats' parade)) of Gion Matsuri in Kyoto. The float in front of us imitates the structure of the compound eye of Drosophila. Each paper lantern resembles an ommatidium, and thorn-shaped ornaments looking like sensory hair are projected from between them. Also, the decoration on the roof of the float also imitates the giant fiber neurons. It was a pity that many summer festivals, including Gion Matsuri, have been canceled or drastically reduced this year, but we hope that they will be splendidly held next year.
Organoids are miniaturized organs cultured in vitro. There are great expectations for its potential, including understanding of the mechanism of organ development, construction of disease models, search for new drugs, and application to regenerative medicine. Now, balloons imitating organoids are flying over the city one after another to announce the advent of a new era. The row of the buildings on the lower right looks like intestinal absorptive cells and Paneth cells lined up. For drawing the balloons, we referred to the following papers: From left, cerebral neocortex (Seto and Eiraku (2019) Neurosci. Res. 138, 33-42), intestine (Lancaster and Knoblich (2014) Science 345, 1247125), and optic cup (Sasai et al. (2012) Development 139, 4111-4121).
オルガノイドは試験管内で培養されたミニ臓器である。臓器の発生メカニズムの理解、疾病モデルの構築、新規薬剤の探索、再生医療への展開など、その可能性に大きな期待が寄せられている。今、新時代の到来を告げに、オルガノイドを模した気球が街に次々と飛来している。下方右の建物の並びは、腸の吸収細胞とパネート細胞が並んでいるさまに見える。気球の作画にあたり、以下の文献を参考にした。左から大脳新皮質(Seto and Eiraku (2019) Neurosci. Res. 138, 33-42)、腸(Lancaster and Knoblich (2014) Science 345, 1247125)、眼杯(Sasai et al. (2012) Development 139, 4111-4121)。
A kingfisher holding an aluminum ion flies toward hydrangea (Hydrangea macrophylla). Hydrangea flowers have a wide variety of colors, but the pigment is the same, delphinidin 3-glucoside, in both red and blue flowers. One of the keys that makes flowers blue is aluminum ions, and it is believed that the formation of a complex between delphinidin 3-glucoside, aluminum ion and copigment (e.g. 5-O-caffeoylquinic acid) results in blue flowers (Ito et al., (2018) Molecules 23, 1424). Three aluminum transporters have been identified in hydrangea so far, one of which was reported in Genes to Cells (Negishi et al., (2013) Genes to Cells 18, 341-352).
アルミニウムイオンを咥えてアジサイ(Hydrangea macrophylla)へ向かって飛ぶカワセミ。アジサイの花の色は多彩であるが、赤い花でも青い花でも、色素は同一のデルフィニジン 3-グルコシドである。花が青色になる鍵のひとつはアルミニウムイオンであり、デルフィニジン 3-グルコシドとアルミニウムイオンと助色素(例えば5-O-カフェオイルキナ酸)が複合体を形成することで花が青色を呈すると考えられている(Ito et al., (2018) Molecules 23, 1424)。アジサイのアルミニウム輸送体はこれまでに3つ同定されており、そのうちの1つはGenes to Cells誌上で発表された(Negishi et al., (2013) Genes to Cells 18, 341-352)。
Each picture in the pattern on this woman's kimono is a cryo-electron microscopy image of E. coli chaperonin GroEL trapped in a thin film of amorphous ice. The method for reconstructing a three-dimensional structure as depicted in this fan from the average of a large number of such images of particles in various orientations (single-particle analysis) continues to improve. We referred a paper by Xu et al. (2016) PLOS ONE, 11(12): e0167765, DOI: 10.1371/journal.pone.0167765 in drawing the patterns on kimono.
この女性の着物の柄のひとつひとつは、大腸菌のシャペロニンGroELをアモルファス氷の薄膜に閉じ込め、クライオ電子顕微鏡で撮影した像である。このような様々な向きの多数の粒子の像の平均から、この団扇に描かれているような三次元構造を再構成する方法(単粒子解析法)が進歩を続けている。着物の紋のデータはXu et al. (2016) PLOS ONE, 11(12): e0167765, DOI: 10.1371/journal.pone.0167765 を参考にした。
The cherry blossom season has come. The latest product of this potted plants selling man is a potted cherry tree in full bloom of unusual design. The pots and trees seem to mimic magnetic beads and antibodies, respectively. They look just like immunoprecipitated cherry blossoms.
In Japan, there is a custom of displaying Hina Dolls on stair-shaped stages at the Doll's Festival on March 3. This year, our dolls are a bit unusual, with the three court lady dolls imitating a trimeric G protein. The current arrangement shows the situation where the α subunit has taken GTP form and has been dissociated from the βγ complex by receiving a signal from the upstream seven, not five, court musician dolls (seven-pass transmembrane receptor).
Attention has been focused on a phenomenon in which the properties of cells are changed by receiving extracellular vesicles containing miRNA and so on. Now, clouds like extracellular vesicles containing miRNA are released from the body of the dragon and flow towards Mt. Fuji. The dragon aims to change the nature of Mt. Fuji. How will Mt. Fuji change by receiving extracellular vesicles?
A demon is cornered by a mouse, the Chinese zodiac symbol of this year, on a neuron-like pole. Serotonin is one of the neurotransmitters that are known to regulate aggressive behavior in animals. According to the classical hypothesis, decreased serotonin levels are associated with increased aggression. However, it is not so simple because there have been opposite reports of increased aggression despite of increased serotonin levels. Further research into the mechanism is underway, and new discoveries are being made.
At a poster session. A crowd is gathering around one presenter who is playing a movie on a tablet. Everybody stares at the movie. I wonder what he is showing. Something hard to resist. If only the tablet were facing this way.
Several types of super-resolution fluorescence microscopies have been invented and begun to spread. One of them is the stimulated emission depletion (STED) microscopy, and its principle is illustrated in this drawing. In the traditional fluorescence microscopy, it is difficult to observe in sub-diffraction limit resolution, since there is a limit to reducing the spot size of excitation beam due to the diffraction limit. On the other hand, a STED microscope can narrow the excited area enough by overlapping the excitation beam and doughnut-shape STED beam. In this drawing, the woman's two umbrellas emit the excitation beam (cyan) and STED beam (orange) overlapping each other, and the fluorescence (green) is emitted from the area much narrower than the excitation beam spot.
ニューロンと グリア細胞の もみじかな
Variously colored maple leaves decorate the autumn season in the mountains overlooking a village. Oh, no, wait a minute, actually they are neurons (red leaves) and astrocytes (yellow leaves) of the central nervous system. What look like branches seem to be blood vessels. In addition, oligodendrocytes and microglia can be seen.
A cluster of butterflies with eyespots on their wings fly away in the sky with concentric circles. Looking carefully, they consist of individuals with wild-type eyespots and mutants with different patterns than the wild-type. There are various theories about the mechanism of forming eyespots. One of them is that it involves the concentric diffusion of a morphogen from the center, but it has recently begun to be questioned. We thank Dr. Shigeyuki Koshikawa (Graduate School of Environmental Science, Hokkaido University) for providing us an idea of drawing and the current status of research in this field.
People have long loved ghost stories which send a chill down their spine in the hot summer season. What is illustrated here is the Chromatin Monster. As a result of tails of histones that form the neck being acetylated, the neck was extended, and it was expressed, scratch that, appeared from an old well while exhaling a spooky breath like mRNA from the mouth.
A method has been spreading for analyzing DNA fragments from organisms in the environment such as water or soil (environmental DNA or eDNA) to identify species being present there and to estimate their population. In this drawing, men are collecting the river water and amplifying certain DNA sequences using a handheld real-time PCR machine to estimate the population of a certain species of fish.
A woman wearing an ornamental hairpin, or kanzashi, likened to L-serine, is looking at the mirror. A D-serine kanzashi on her hair can be seen in the mirror. Since it has been revealed that at least some D-amino acids play physiological roles, focusing only on L-amino acids might be 'out of fashion.'
Many people come and go across a bridge in Kyoto. Two mountains, or actually histograms can be seen at the back. These graphs are the distribution of sleep amount for male (front, 'XY') and female (back, 'XX') flies of a large number of strains in a Drosophila mutant library, and the horizontal and vertical axes represent sleep amount and frequency (the number of strains), respectively. Since many strains showed longer sleep in male than in female in this experiment, a divergence can be observed between the peaks of the histograms. For drawing this image, we referred to Cirelli et al. (2005) Nature 434, 1087-1092.
京の都の橋の上を人々が行き交う。背景に2つの山、いやヒストグラムが見える。これらはショウジョウバエの変異体ライブラリーに含まれる多数の系統で睡眠時間を測定し、雄(手前、XY)と雌(奥、XX)に分けてプロットしたもので、横軸が睡眠時間を、縦軸が度数(系統の数)を示す。この実験では雄の方で睡眠時間が長めの系統が多かったので、ヒストグラムのピークにずれが生じている。作図にあたり、Cirelli et al. (2005) Nature 434, 1087-1092を参考にした。
The single-cell RNA-sequencing technology has a possibility of changing biological studies completely. This is a schematic image of the technology. As with petals of the cherry blossoms, for example, even a number of early embryonic cells indistinguishable from each other can be clustered and visualized based on gene expression data, just as the image is projected on the water surface, and then further analysis can be progressed. Data: Dr. Takefumi Kondo and Mr. Shunta Sakaguchi (Kyoto University).
This is the 'Spindle Checkpoint' placed at the important point 'Metaphase-Anaphase Transition' in the post town 'M Phase' on the 'Cell Cycle Highway.' The mitotic spindles and chromosomes carried by the travelers are carefully inspected, and the travelers must be arrested until all the chromosomes have been aligned at the equator and all the pairs of sister kinetochores have been properly attached to the microtubules from both poles of the spindle to achieve bi-orientation. Only those who safely pass the checkpoint can enter the 'anaphase' of their trips.
One winter day when the snow covered the trees and the ground, a woman is taking out racks, in which frozen samples are orderly stored, from a liquid nitrogen cryogenic storage vessel with a dipper. There might not have been any satisfactory safety gloves at that time. Patterns of snow crystals are printed on her kimono sash.
Kagami-mochi (mirror rice cake) is a representative of Japanese New Year decorations. It usually consists of a couple of stacked large round-shaped mochi that resemble ancient bronze mirrors with one daidai (Japanese bitter orange) on top. This year, however, our kagami-mochi is somewhat different from the usual; it is a so-called ribosome-like kagami-mochi. Two stacked mochi likened the ribosome's large and small subunits bind to a gohei (a folded ribbon of paper) that resembles an mRNA encoding (DAIDAI)n amino acid sequence, and a chain of daidais extends upward from the mochi likened the large subunit. It looks like a polypeptide chain itself.
Mochi (rice cake) is indispensable to welcome the New Year in Japan. To use mochi for various purposes such as for food and for ornament, people firstly pound a large mochi and then divide it into small pieces according to the purposes. This process may be similar to that of producing various types of cells from fertilized eggs by asymmetric cell divisions. The woman in the front is dividing a large mochi asymmetrically such that beans that look like Sara (Smad anchor for receptor activation) endosomes and a crescent that looks like Numb (a cell fate-determining factor) are gathered only into the left half. The other woman on the back is preparing a decoration called mochi-bana (rice-cake flower). Note that she is trying to schematize a linage of stem cells and progeny. For drawing this image, we referred to Gonzalez (2015) Nature 528, 196-197, DOI: 10.1038/528196a.
日本の迎春に欠かせないのが餅である。食用や装飾用などさまざまな用途に利用していくために、まずは大きな餅をついてから、目的に応じて小さく分割していく。そのやり方は、細胞の非対称分裂によって受精卵からさまざまな種類の細胞が形成されていく過程に似ているかもしれない。手前の女性は大きな餅を分けようとしているが、ちょうどSara(Smad anchor for receptor activation)エンドソームのような豆粒と、三日月状の運命決定因子Numbのような三日月状の部分が左側の餅だけに非対称に集まるようにしている。奥の女性は餅花を作っているが、幹細胞と子孫細胞の系譜を模式的に表そうとしていることに注意してほしい。作画においてGonzalez (2015) Nature 528, 196-197, DOI: 10.1038/528196aを参考にした。
When drawing a saturation binding curve of two proteins, there is a way of plotting the concentration of the free form of one of the pair on X axis, and the ratio of the other that is forming complex on Y axis. In this case, the slope of the curve is small if their affinity is low (i.e. large dissociation constant (Kd)); however, the slope is large if the affinity is high (i.e. small Kd). In this drawing, two flocks of wild geese are flying along two line-shaped clouds that are likened to saturation binding curves. The geese along the lower, gentle-slope cloud are flying apart as if their affinity is low. On the other hand, the ones flying along the steep-slope cloud in front of the moon are gathering together as if their affinity is high. For drawing this image, we referred to the research paper by Sadaie et al. (2014) Mol. Cell. Biol., 34:3272-3290, DOI: 10.1128/MCB.00087-14 that reports in vivo Kd values.
2つのタンパク質間結合の飽和曲線では、X軸に片方のタンパク質の遊離濃度を、Y軸にもう一方のタンパク質が複合体を形成している割合を示すことがある。その場合、両者の親和性が低い(解離定数Kd が大きい)ときは曲線の傾きが小さいが、親和性が高い(Kd が小さい)ときは曲線の傾きが大きい。この絵では、飽和曲線に模した2筋の線状の雲に沿って2つの雁の群れが飛んでいる。下方の、傾きの小さい雲に沿って飛ぶ群れは、まるで互いの親和性が低いように分散して飛んでいる。一方、大きく傾き月の手前を横切っている雲に沿う群れは、親和性が高いかのように互いに密着している。作画にあたり、in vivoでのKd 値を報告しているSadaie et al. (2014) Mol. Cell. Biol., 34:3272-3290, DOI: 10.1128/MCB.00087-14 を参考にした。
There is a pedestrian suspended bridge without side guards over a ravine surrounded by deer on the mountains and birds in the sky. The bridge bent by the weight of two persons, and its fork just look like the replication fork of DNA. The couple is walking across the bridge at the very point of the fork as if they were the replication machinery.
Katsushika Hokusai published not only ukiyo-e pictures, but also Hokusai Manga (Hokusai's Sketches) in which he presented how to draw assorted geometric patterns in addition to various examples of landscapes and figures of humans, animals, plants and so on. We drew Seigaiha (blue ocean wave) with auxiliary lines on the background of this drawing, and found it astonishingly close to the patterns of fish scales. Hokusai might have known it. For drawing this image, we referred to the research paper by Aman et al. (2018) eLife, 7:e37001, DOI: 10.7554/eLife.37001 that reported development of scales.
葛飾北斎は浮世絵だけではなく、スケッチ集である『北斎漫画』を出版していた。北斎漫画には、様々な風景・人物・動植物などの作画例に加えて、さまざまな幾何学的文様の作図方法も示されている。この画の背景には、その文様のひとつである青海波(せいがいは)を、補助線を消さずに描いているが、これは魚のウロコの配列に驚くほど似ている。北斎はもしかするとそれに気づいていたのかもしれない。作画にあたり、ウロコの発生を報告したAman et al. (2018) eLife, 7:e37001 DOI: 10.7554/eLife.37001を参考にした。
Fireworks likened to newly generated neurons have been launched from the brain. In recent years, it has been shown that neurons are continually generated in the brain of adult mammalians. At the very moment when these pyrotechnic 'stars' are engraved on your brain as an unforgettable memory of the summer, your new neurons should be working. We thank Dr. Itaru Imayoshi (Kyoto University) for providing the data.
A row of houses and a mountain can be seen behind rafts for cultivation of laver lined up in parallel. It just looks like a visualization of RNA-seq data mapped to a reference genome in order to see the depth of coverages (mountain) of reads (rafts) around a certain exon (row of houses) . We thank Dr. Fumiki Katsuoka (Tohoku Medical Megabank Organization, Tohoku University) for providing such a splendid idea for us.
整然と平行に並ぶ海苔養殖の筏の並びのちょうど後方に家並みと山が見える。そのさまは、リファレンスゲノム配列にマップしたRNA-seqのデータを、あるエキソン(家並み)とその周辺でのリード(筏)のカバレッジ深度(山)を見るために可視化したさまに似ている。アイディアを提供してくださった東北大学 東北メディカル・バンク機構の勝岡史城博士に感謝申し上げる。
At first glance, it might seem to be a Japanese rock garden (karesansui) with wavy lines in the sand (samon); however, it is actually an electron microscopic photograph of Drosophila midgut epithelium taken with the freeze-fracture method. The lines that look like samon are smooth septate junctions, which are a kind of occluding junction seen in invertebrates. We thank Prof. Mikio Furuse and Ms. Kyoko Furuse (National Institute for Physiological Sciences) for providing the data and sharing the idea of karesansui.
波状の美しい砂紋が施された枯山水の庭。と思いきや、実は凍結割断レプリカ法で撮影したショウジョウバエの腸管上皮の電子顕微鏡写真であった。砂紋のように見えるのは、無脊椎動物でみられる閉鎖結合の一種であるスムースセプテートジャンクションである。データをご提供いただき枯山水のアイディアを共有していただいた古瀬幹夫教授と古瀬京子氏(自然科学研究機構 生理学研究所)に感謝申し上げる。
This is an acrobatic performance imitating immunoprecipitation using a phosphorylated protein-specific antibody. Two boys are acting two forms of a substrate protein. The boy on the left who wears red trousers, that represent the phosphorylation site of the substrate protein, is showing an opened fan and striking a pose that looks like the active form of the substrate protein. The other one who wears blue trousers is squatting on the ground to play the role of the dephosphorylated, inactive form of the substrate protein. The man who is suspended upside down in the midair is acting a phospho-specific antibody, and grasping the red trousers only, i.e. the site of phosphorylation.
A female weaver at the loom is dexterously managing a shuttle likened the condensin complex and a heddle with a weight likened to the cohesin complex as if they were parts of her own body to weave a metaphase chromosome-patterned fabric.
Researchers measured the concentration of various metabolites in plasma or red blood cells taken from volunteers, and found metabolites whose concentration was specifically higher or lower in elderly individuals. In this picture, two researchers are spreading these compound structure formulae beside them, and discussing correlations of concentration between two metabolites by plotting scatter charts using a lattice of a shoji door. A bamboo fence that is likened to a clustered heatmap of metabolites can be seen over there. For drawing this image, we referred to a research paper by Chaleckis et al. (2016) Proc. Natl Acad. Sci. USA, vol. 113, pp. 4252-4259. DOI: 10.1073/pnas.1603023113.
さまざまな代謝産物の血漿中・赤血球内の濃度をボランティアにおいて測定し、高齢者特異的に濃度が高まっている、あるいは下落している代謝産物を見いだした。この画では、研究者らがそれらの化合物の構造式を手に、2つの代謝産物の濃度間の相関を、障子の格子を利用して散布図に表して議論している。奥には、代謝産物をクラスタリングしたヒートマップに擬せられた竹垣が見える。散布図の作画にあたって以下の論文を参考にした。Chaleckis et al. (2016) Proc. Natl Acad. Sci. USA, vol. 113, pp. 4252-4259. DOI: 10.1073/pnas.1603023113
In Japan, people cerebrate Setsubun, the day before the lunar calendrical beginning of spring, around 3rd of February every year. People decorate sardine heads and holly leaves, put soy beans in a square wooden measuring box ('masu'), and scatter the beans to expel the bad spirits. Putting not only ordinary soybeans, but also other kinds of beans, we expressed a scene of the inside of a cell in the masu just like a bonsai. The real inside of the cells isn't sparse, but very much crowded, like this masu, with cellular organelles such as the nucleus, mitochondria, secretory vesicles and Golgi apparatus and cytoskeletons as well. For drawing this image, we referred to an essay by Shoichiro Tsukita
At Japanese shrines during New Year's holidays, you can find such dedicatory barrels of sake as in this picture. Looking closely at their labels, you will see the 3D structure, drawn on them, of green fluorescent protein ('緑乃光'), chaperonin ('寫丙論' and '界添'), proteasome ('蛋白ごろし'), aquaporin ('水之路') and sucrose porin ('満久淡泊'), all of which have barrel-like tertiary or quaternary structure.
You can perform polymerase chain reaction (PCR) without using a thermal cycler if you follow this drawing. Time management and temperature control are critically important for getting good yields of reaction products with high reproducibility and specificity. But, of course, safety first. Ouch! The smoke got in her eyes!
The position of the hindlimb, or the length of the trunk, of tetrapods is highly diversified. Very recently, it was revealed that the timing of expression of Gdf11 gene in tetrapods' embryonic development plays an important role in determining the eventual position of the sacral vertebrae and the hindlimb beyond species. In some species that has a short trunk, such as frog, Gdf11 is expressed relatively earlier; in contrast, it is expressed much later in snakes' embryo. On the cover of this issue, we drew tetrapods with various lengths of the vertebrae, imitating Choju-jinbutsu-giga, one of Japan's national treasures, on a photograph of bamboos, which remind us of somites. For drawing this image, we referred to a website and a research paper by Matsubara et al. (2017) Nature Ecology and Evolution, vol. 1, pp.1392-1399, DOI: 10.1038/s41559-017-0247-y.
四肢動物の後肢の位置、すなわち胴の長さは多様性に富んでいる。ごく最近、四肢動物の胚発生におけるGdf11遺伝子の発現のタイミングが、種を超えて仙椎と後肢の位置の決定に重要な役割を果たしていることが明らかとなった。カエルのように胴の短い種ではGdf11が早いタイミングで発現するのに対し、ヘビの胚ではその発現のタイミングが非常に遅い。今号の表紙画では、体節を想起させる竹林の写真に、日本の国宝である鳥獣人物戯画に似せた筆致で様々な長さの脊椎を持つ四肢動物たちを描いた。作画にあたって以下のWebサイトと論文を参考にした。 および Matsubara et al. (2017) Nature Ecology and Evolution, vol. 1, pp. 1392-1399, DOI: 10.1038/s41559-017-0247-y.
中心体の大名行列 気づいておくれよ脱重合
In the Edo period, feudal lords in Japan ('daimyo') had to travel back and forth between their lands and Edo (present-day Tokyo) every other year. It must have been hard for the vassals and retainers accompanied on foot. In this drawing, one prominent daimyo and his procession are depicted. The daimyo's family crest is modeled on the centrosome that acts as the main microtubule organization center in animal cells. The procession is preparing for departure from a 'honjin' (accommodation for daimyo) before dawn. It is still so dark that nobody seems to have noticed that the tips of the pole-like equipment made of microtubules have started depolymerization. They would completely depolymerized on the road unless somebody caps them with GTP-tubulins before departure.
A flock of wild geese from the north seems to be flying in front of the autumn moon rising behind a place of scenic beauty. No, it’s not. Looking carefully, it is a shoal of sperms swimming toward an ovum. It seems that one sperm has reached the ovum and passed through the zona pellucida, and that fertilization has just taken place.
Mutations in PARKIN or PINK1 genes can cause autosomal recessive juvenile Parkinson's disease. In recent years, it has been revealed that they participate in mitochondrial quality control. Their products, Parkin and PINK1, modify dysfunctional mitochondria with polyubiquitin chains to promote selective autophagy (mitophagy) that eliminates dysfunctional mitochondria from cells. The woman in this drawing is removing unwanted seeds from the watermelon just as mitophagy eliminates dysfunctional mitochondria.
Kyoto's Gion Matsuri is one of Japan's three greatest festivals, and its highlight is Yamahoko Junko (decorated floats' parade) in which many decorated floats with hundreds of years of tradition parade through the central part of Kyoto. Now, is the float passing by in front of us Naginata-boko that is famous for its naginata (long-handled sword) on top? No, it is the 'telomere' float. In the T-loop-like structure of the telomere extending upward on the roof, you can see two Shagumas (straw ornaments) likened to TRF1 and TRF2 bound to the double strand region and Ohata (flag) likened POT1 bound to the 3′-single-stranded overhang. These factors are comprised of the shelterin complex and need to work cooperatively and continuously for maintenance the telomere structure. Such unremitting efforts are needed to preserve not only traditions, but also telomeres.
Fireflies are flying and glowing fantastically. Patterns of firefly squids, 'yakoutake' (glowing mushrooms; Mycena chlorophos) and 'owankurage' (crystal jelly; Aequorea victoria) are seen on kimono. They share a common mechanism in which a class of chemiluminescent substrate (luciferin) is oxidized and excited by the catalytic function of a class of enzymes (luciferase), and light is emitted as a result. There has been a custom of appreciating the glow of fireflies in Japan since ancient times, however, it was possible only for a short period in early summer. Life scientists today are lucky to admire the chemiluminescent signal throughout the year. The structural formulas in the sky, and on the woman's and child's round fans are those of firefly luciferin, its dioxetane derivative and excited oxyluciferin, respectively.
幻想的な光を放ち飛ぶ蛍と、着物に描かれたホタルイカ・ヤコウタケ (Mycena chlorophos)・オワンクラゲ (Aequorea victoria) たち。これらが光を生み出すメカニズムは共通しており、いずれも発光基質(ルシフェリン)が酵素(ルシフェラーゼ)の触媒作用で酸化されることで基質が励起する結果、光が放出される。日本では昔から蛍の光を愛でる習慣があったが、初夏の一時期に限定されていた。現在の生命科学者は、幸運なことに一年を通じてその化学発光を愛でているわけである。背景の空、女性のうちわ、子のうちわにある構造式は、それぞれ蛍のルシフェリン、そのジオキセタン誘導体、励起されたオキシルシフェリンのものである。
May in the old Japanese luni-solar calendar is a season when flowers of 'hanashobu' (Japanese iris, Iris ensata var. ensata) are in full blossom by the waterside. In the pond in this garden, clusters of hanashobu can be seen. Oh, watch carefully! Some flowers in front of us are made of α-helices and β-sheets, aren't they?
旧暦5月は水辺に花菖蒲(Iris ensata var. ensata)が咲き誇る季節である。この庭園の池にも群生している花菖蒲が見られる。おや? よく見てみると、手前の何本かの花は、αヘリックスとβシートで出来ているのではないか?
Today, more than one fourth of people living in Japan are estimated to be suffering from 'sugi' or Japanese cedar (Cryptomeria japonica) pollinosis. Spring should be the worst season for those with such allergies. Binding of the allergen to immunoglobulin E (IgE) on the surface of mast cells triggers release of histamine from them, and it causes symptoms such as itchy eyes or snivel. However, it's not a problem only for human beings. This monkey performing saru-mawashi (monkey show) also has a pollen allergy, and today's stage is, of all places, beside the very sugi tree!
We found a spray of ume (Japanese apricot) with some pretty blossoms. To bring out the best appearance of the blossoms, we arranged it in front of a plate on which a budding yeast strain had been streaked. Since the strain had ade2 mutation and carried a plasmid containing ADE2 gene, a moderate number of colonies turned into deep pink color due to loss of the plasmid were scattered on the medium. What we found as a result was a perfect picture of ume blossoms illuminated from behind by the rising full moon.
Walking monozygotic twin sisters in the snow are sharing an umbrella. They share the same genotype; however, they seem to have a different personality and taste as their kimono. It has been pointed out that the difference in epigenome would cause such difference in personality. The data printed on their sleeves that was obtained with the bisulfite sequencing (a method that can detect methylation of individual cytosine residues in DNA) and is showing their methylation patterns at a certain locus of the genome suggests that there are epigenetic differences between them. Our knowledge of the relationship between presumed hereditary traits or diseases and epigenetics will increase through studies of monozygotic twin-pairs of which one twin has such traits or diseases.
In recent years, sirtuin family genes have attracted attention because it has been reported that their activation extended lifespan in some animal species and yeast. Sirtuin family consists of several classes, and the majority of them encode NAD+-dependent deacetylase. The arrow in this picture is called 'hamaya,' which is one of Japanese New Year's good luck charms. It is decorated with two 'ema's (wooden votive plaques) likened five-membered ring of ribose with drawings of a crane and a turtle, common symbols of longevity in Japan. The arrow as a whole is mimicking the structure of NAD+, which is required for sirtuin's deacetylase activity.
A woman is dispensing reagents using a motorized pipettor. She is in a stylish kimono (hogusome) with a pattern like letters written with a brush. The text on her kimono is not only for fashion but also actually the recipe for the reagents (NaCl, glucose, amino acids, acetic acid, etc.); that is exactly to kill two birds with one stone. It will help her do experiments precisely at the busy end of the year.
女性が、電動ピペッターを用いて試薬を分注している。彼女が着ているのは、筆文字のような粋な柄(反古染(ほぐそめ))の着物。着物に書かれている文字は、単におしゃれのためではなく、実は試薬のレシピ(NaCl, グルコース, アミノ酸, 酢酸など)にもなっており、まさに一石二鳥だ。師走の慌ただしい中の実験も、これで大丈夫。
It is known that an increase of cytosolic concentration of calcium ion (Ca2+) like a wave is initiated from the point of sperm entry and propagates throughout the egg after fertilization in many animal species. This calcium wave is caused by a kind of chain reaction; activated phospholipase C (PLC) produces inositol 1,4,5-triphosphate (IP3) that induces release of Ca2+ from the endoplasmic reticulum (ER) near the egg surface, and released Ca2+ induces further release of Ca2+ from nearby ER. The leaves on the round-shape mountain are turning red like a wave coming down from the top of the mountain as if mimicking the calcium wave.
Site-specific DNA recombination technologies such as FLP-FRP and Cre-lox systems have been applied to various purposes including mosaic analysis or conditional knockout. We applied the mechanism to pruning a pine tree. When we gave a signal to the pine tree, a branch was circularized and was cut automatically at the specific site marked with the darker green pine needles.
Soba (buckwheat, Fagopyrum esculentum) is a pseudocereal with rich aroma and flavor, which is mainly eaten as noodles after Edo era in Japan. Yet there still remain many problems to overcome: its lower yield than the wheat or rice mainly caused by heteromorphic self-incompatibility (SI), its allergenic property that sometimes causes severe allergic reactions, and so on. Also, SI has been preventing us from producing good cultivars using genetic approaches. Finally, the buckwheat's whole genome has just been sequenced with next-generation sequencing (NGS), and it shows us a solution for those problems. We may have low-allergenic soba noodles in the foreseeable future.
ソバ(Fagopyrum esculentum)は豊かな香味を有する重要な擬似穀類であり、日本では江戸時代以降、主に麺として食されている。しかし、主に異形花型自家不和合性(SI)のために小麦や米に比べ収穫量が少ないことや、時に重篤なアレルギー反応を引き起こす問題など、改善すべき点が残っている。またSIのために、遺伝学的アプローチによる品種改良には困難が伴う。最近、そのソバの全ゲノムが次世代シーケンシング(NGS)により解読され、それらの問題の解決の糸口が見えてきた。そう遠くない将来、低アレルゲン性の蕎麦を食べられるようになるかもしれない。
An infant and his mother are playing with a small fish in a tub. The tub has a knothole from which water is pouring out, so the infant is carefully holding the small fish not to let it out from the hole. In the plasma membrane in various species, from bacteria to higher eukaryotes, there are such water channels called aquaporins that selectively allow water molecules to traverse the membrane. The schematized secondary and tertiary structures of the aquaporin's monomer are drawn on his mother's kimono and sash, respectively. And, aquaporins form tetramers to function in the plasma membrane just like the pattern drawn on the infant's kimono.
Naruto Strait, a strait between Shikoku and Awaji-shima Island, is famous for the huge Naruto-no-uzushio (Naruto whirlpools) that is generated by Japan's fastest current of sea water caused by the tide. It is thanks to the function of our inner ears that we can hear the roaring sound from and feel the acceleration of the rotation of the whirlpools. We saw an illusion of the structure of the inner ear (the semicircular canals, vestibule and cochlea) in a huge whirlpool that shook us by the inner ears.
Among phenotypes, quantitative traits are usually caused by many chromosomal loci (quantitative trait locus, QOL) in a complex manner, and researches using dogs have been fruitful. A number of dog breeds have been produced by human beings. The morphological character is highly variable among breeds, but uniformed within the same breed. Genome-wide association studies that coupled craniometric data obtained from various dog breeds have been conducted, and QTLs in which SNPs that might affect the skull shape existed concentratively were identified. One of the QTLs was mapped near BMP3 gene on Ch. 32, and it was shown that most breeds with shortened head had a common missense mutation in BMP3. Data on the background: Figure 3 of Schoenebeck et al. (2012) PLoS Genet. 8, e1002849 (DOI: 10.1371/journal.pgen.1002849). Upper panel: SNP distribution around BMP3 locus among various dog breeds. Lower panel: Plots of important SNPs (conserved, exonic, etc.) extracted from the upper panel.
生物が示す形質のうち、量的形質には染色体上の多数の座位(量的形質座位、QTL)が複雑に関与していることが多いが、イヌを用いた研究が成果を上げている。人間の手により数多くのイヌの品種が作出されてきた。イヌの形態的特徴には大きな品種間差異がある一方で、品種内では均質である。さまざまな品種の頭骨の形状を測定した結果を加味したゲノムワイド相関解析が行われ、頭骨の形状に影響すると推定されるSNPが集中しているQTLが同定されている。そのひとつは第32番染色体上のBMP3(bone morphogenetic protein 3)遺伝子付近にマップされ、短頭犬種の多くがBMP3のミスセンス変異を共通して持つことが示されている。背景のデータ出典:Figure 3 of Schoenebeck et al. (PLoS Genet. 8, e1002849 (2012). DOI: 10.1371/journal.pgen.1002849)。上段:BMP3遺伝子周辺のさまざまな犬種のSNP分布、下段:上段のSNPのうち、よく保存されている・BMP3のエキソン内に存在するなど、重要なものを抽出し図示したもの。
Flow cytometry is a technology that allows high throughput analysis of the characteristics of cell populations by optically and rapidly measuring the morphological characteristics and fluorescent signals of the cells suspended in a narrow stream of fluid in equipment (flow cytometer). In addition, the equipment with a cell sorter can collect a certain type of cells from a heterogeneous population by charging droplets containing such cells identified by the optical data and attracting them by an electrostatic deflector. In this post town in this drawing, 'Positive' inn on the right and 'Negative' inn on the left are finding and attracting potential customers from passersby. They just look like a cell sorter. There is seen above them a cluster of clouds that resembles flow cytometric data.
Kiyomizu-dera temple in Kyoto is famous for its 12-meter high Butai ('stage'). In Edo era, there was a popular superstition that if one survived a jump from the Butai, one's wish would come true. In this drawing, one woman holding an umbrella as a parachute has jumped from the Butai over the fully blossomed cherry trees. In the Drosophila wing imaginal discs drawn on her kimono, the spatial expression patterns of genes involved in pattern formation can be discerned. May the Drosophila wing imaginal discs differentiate into wings before she crashes to the ground. Drosophila adults have gathered from nowhere as if they escorted her in a cherry blossom snowstorm.
Spring, that is the season of arousal for many living creatures. Even among mammals, some squirrels, bears and bats hibernate. This woman is researching hibernation of an individual of the Siberian chipmunk. The body temperature of hibernating chipmunk firstly drops to near ambient temperature, and reciprocally repeats the state of less than 10 °C (sustained hibernation) and the state of almost 37 °C (inter-bout arousal) like the chart on this drawing. We hope that life science will shed light on the mechanisms of various environmental adaptations of life. We thank Dr. Yoshifumi Yamaguchi (Graduate School of Pharmaceutical Sciences, The University of Tokyo) for his valuable suggestions for our drawing. Inspired by Figure 1 in Epperson & Martin (2002) Physiol. Genomics 10, 93-102.
In the most popular type of next-generation DNA sequencer, DNA fragments are PCR-amplified in the slide's channel coated by a lawn of PCR primers to form myriads of clone DNA clusters. After that, the DNA sequence of each cluster is determined by taking photographs of fluorescent signals on the slide with the image sensor again and again after each single-nucleotide extension step of a novel dye-terminator method. Thus, the reaction proceeding in the next-generation sequencer is massively parallel sequencing. In this drawing, we schematically expressed this mechanism with a flying hawk (image sensor) viewing clusters of pine trees (DNA) on the channel-shaped reclaimed land divided by embankments.
現在最もよく普及しているタイプの次世代シーケンサーでは、スライドガラス上のPCRプライマーのローンに覆われたチャンネルの中でDNA断片をPCR増幅させ、無数のクローンDNAのクラスターを形成させる。次いで、新規のダイターミネーター法で1塩基伸長するごとにスライドガラスの蛍光像をイメージセンサーで撮影することを繰り返すことで、それぞれのクラスターのDNA配列が決定される。すなわち次世代シーケンサーの中で起きている反応は、massively parallel sequencingである。この画ではそのしくみを、チャンネルのような土手と土手で囲まれた広い溝状の造成地にクラスター状に生える松(DNA)を眺めながら空を舞う鷹(イメージセンサー)の様子として模式的に表現した。
Worship the first sunrise of the new year from two rocks ‘Meoto-iwa’ (the wedded rocks, Mie, Japan) under double-helical DNA-like contrails in the sky. From left to right, there is the big rock, the sacred straw rope, the smaller rock, and the multi-exposed chain of suns. They just look like an RNA polymerase transcribing DNA into mRNA, its resultant mRNA, a ribosome translating the mRNA, and the new polypeptide chain growing upward from the ribosome, respectively.
Researchers in a lab at the very last moment on the night before a scientific meeting they are supposed to give their presentation. One is elated that her poster has been already done, while the other three are obviously fretting. The two people in the front are preparing for oral presentations. One on the left front is rehearsing with his slideshow, and the other is still revising her manuscript. Most of all, one on the left back is still collecting data for her slides, yet. Do not leave anything behind you, and good luck on your presentation tomorrow! Inspired by Figures 1(A) and 4(E) in Öst et al. (2014) Cell 159, 1352-1364.
学会前夜の研究室で、ぎりぎりまで発表準備に追われている研究者たち。ポスターの印刷が終わっている者は余裕の表情だが、まだ準備中の三人には焦りの色がありありと見える。手前の二人は口頭発表の準備中。そのうち一人はスライドショーを前に発表練習をしているが、もう一方はまだ発表原稿を直しているところだ。左奥のメンバーに至っては、まだスライドのデータを集めている。皆さん、お忘れ物はないか、出発前に確認を。屏風の柄はÖst et al. (2014) Cell 159, 1352-1364のFig. 1(A)とFig. 4(E)より着想を得た。
The normal white (w+) gene in Drosophila is required for production of red pigment in the eyes. In some mutants in which the w+ gene has been moved to a region near the centrosomal heterochromatin due to an inversion in the X chromosome, the eyes are mottled with red and white patches; the w+ gene in the white cells has been silenced due to spreading heterochromatin, while w+ gene in the red cells is active. This phenomenon is called PEV (position-effect variegation), and a number of factors that control epigenetics, such as histone methyltransferases, have been identified by measuring the extent of variegation. Now, in this sliding screen, maple leaves in autumn were drawn with Japanese ink on gold foil background except some in the central oval with a red paint as an accent. A shadow that looks like Drosophila happened to be cast on it, emerging a figure of the w mutant with a mottled eye.
ショウジョウバエの正常なwhite遺伝子(w+)は眼の赤い色素の合成に必要である。w+遺伝子がX染色体に生じた逆位によってセントロメア近傍のヘテロクロマチン領域の近くに移動している変異株では、眼が赤白のまだら模様になる。w+がヘテロクロマチン化されてサイレンシングされている白い細胞とw+が発現している赤い細胞が生じるからである。この現象は斑入り位置効果(position effect variegation, PEV)と呼ばれ、まだらの程度を指標にしてエピジェネティクスに関与する因子が多数同定された。さてこの襖絵は、金箔の上に秋の紅葉が墨で描かれ、中央付近の楕円の領域の紅葉のみ鮮やかな赤で描かれている。そこに偶然、ショウジョウバエのような影が映った。すると、まだら模様の眼を持つそのw変異株の姿が浮かび上がった。
Kappa, a mischievous monster found in Japanese folklore, supposedly lives in a stream or pond, and has webbed hands and feet so that it's good at swimming. Human hands also have an interdigital web in the early embryo, but it disappears by apoptotic programmed cell death like a tadpole's tail. The intracellular signaling pathway that triggers apoptosis has been studied well; the signal flows down the 'cascade' that consists of multiple caspases (Cysteine-ASPartic proteASE) such as caspase 9 or 3 encoded by CASP9 or CASP3, respectively. This poor Kappa in this drawing has been saturated with the water flowing down the caspase cascade, and its vaunted web is in danger of disappearing by apoptosis.
河童は日本の民話に登場する悪戯好きの妖怪で、川や池に棲み、水かきのついた手足を持っているので泳ぎが得意とされる。人間の手にも初期胚の段階では水かきがあるが、オタマジャクシの尾部と同様に、アポトーシスによるプログラム細胞死により消失する。アポトーシスを引き起こす細胞内シグナル伝達経路はよく研究されており、caspase 9やcaspase 3 (それぞれCASP9遺伝子とCASP3遺伝子にコードされている)といった複数のカスパーゼ(Cysteine-ASPartic proteASEの略)からなる「カスケード」をシグナルは流れ下る。哀れにもこの画の河童は、カスパーゼカスケードを流れ下る水を浴びてしまい、自慢の水かきがアポトーシスで消失しそうになっている。
Shoki (Zhong Kui in Chinese), derived from Chinese Taoism, is a god for warding off diseases. According to a legend, one night when Emperor Genso (Xuanzong) of the Tang Dynasty had a high fever, Shoki appeared to him in a dream, killed a monster, and saved the Emperor at last. In tribute to the legend, Shoki has been worshipped as a god who protects people from illness for hundreds of years in China and Japan. The role of Shoki in the legend is that of the immune system itself. In this drawing, Shoki holds a sasumata (spear fork) with an IgG-like spearhead in the right hand and wears a cloth with a crest of IgM and a pattern of a dendritic cell; they are weapons of mammalian adaptive immunity. In contrast, his left hand contains a monster (pathogen) by melanization, which is one of the weapons of the innate immunity of insects. We thank Prof. Hirotaka Kanuka's lab (The Jikei University School of Medicine) for their valuable suggestions for our drawing.
鍾馗(中国語でZhong Kui)は中国の道教由来の病除けの神である。ある伝承によると、高熱に苦しむ唐の玄宗皇帝の夢枕で鍾馗は鬼を退治し、皇帝を病から救ったという。その伝承にちなみ、鍾馗は病から身を守ってくれる魔除けの神として、中国や日本で何百年にわたり信仰されてきた。この伝承の中での鍾馗の役割は、まさに免疫系の役割そのものである。この画の鍾馗は、右手で先端にIgGを使った刺又を構えており、身にまとう衣装にはIgMの紋と樹状細胞の模様が描かれているが、これらは哺乳類の獲得免疫の武器である。一方、左手では、昆虫の自然免疫の武器であるメラニン化により、鬼(病原体)を封じ込めている。作画に当たり有益な助言をいただいた慈恵医科大学の嘉糠洋陸研究室に感謝申し上げる。
Transposons (transposable elements), discovered in maize by Barbara McClintock in 1948, are DNA sequences that can change their positions within the genome. Transposition of transposons can introduce mutations in the host genome. Actually, there was a close and astonishing relationship between transposons and the culture of the Edo era. In late Edo, a large number of mutant Asagaos (Japanese morning glory) were isolated, and it became popular to cultivate them; in fact, many mutations were caused by transposons. These mutant Asagaos have been collected by researchers and are currently maintained by a group led mainly by Kyushu University. See the website of National BioResource Project (NBRP) “Asagao” ( for further details.
トランスポゾン(転移因子)はゲノム内で位置を変えるDNA配列で、1948年にバーバラ・マクリントックによりトウモロコシで発見された。トランスポゾンの転移はホストゲノムに突然変異を引き起こすことがある。実はトランスポゾンと江戸時代の文化には深い、驚くべき関係があった。江戸時代後期、様々な変異型アサガオが単離され、その栽培が流行したが、実は多くの変異の原因はトランスポゾンだったのである。これらの変異型アサガオは研究者により収集され、現在では九州大学を中心としたグループにより維持されている。詳しくは、ナショナルバイオリソースプロジェクト「アサガオ」のWebサイト を参照。
One of the highlights of the month of July in Japan is Tanabata (Star Festival). Tanabata, a festival to pray to the stars in the Milky Way in summer, was imported from ancient China. People in Edo era started to decorate bamboo branches with ‘tanzaku’ (small pieces of paper) on which they wrote their wishes, and to pray the wishes would come true. The bamboo branches in this drawing are likened to histone tails (H2A, H2B, H4 and H3 from the left). The tanzakus represent epigenetic modifications (acetylation, methylation and phosphorylation) of amino acid residues in histone tails. If you use this histone bamboo, mind that you have to phrase your wishes with the types, positions and combinations of decorations (or modifications). You need a textbook to perform modifications correctly.
7月の日本の見どころは七夕(星祭り)である。七夕は、古代中国が起源の、夏の天の川の星々に祈りを捧げる祭りである。日本では江戸時代から、人々は七夕に、笹の枝に願いを書いた短冊(紙の小片)を飾り、願いが叶うことを祈るようになった。この画の笹の枝はヒストンテール(左から順にH2A, H2B, H4, H3)を模している。短冊はヒストンテールのアミノ酸残基へのエピジェネティックな修飾(アセチル化、メチル化、リン酸化)を表している。もしこのヒストンの笹を使うなら、願い事の内容は、飾り(すなわち修飾)の種類と位置、および修飾の組み合わせで表現せねばならないので注意が必要だ。正しく修飾をするには、教本が欠かせない。
It was in 1936 when the existence of ‘florigen’ was initially predicted as a plant hormone that transmits information on daylight length at their leaves to the tip of the stem and stimulates flower initiation. In 2007, it was finally identified as a protein, which is coded by the FT gene in Arabidopsis thaliana. FT protein is produced in the leaves and transmitted to the growing tip at the shoot apical meristem via the phloem, and interacts with a transcription factor (the product of the FD gene in Arabidopsis) to induce genes involved in flower initiation. In this drawing, an actor performing FT is contacting another actor performing FD, and trying to stimulate flower initiation with him in front of hydrangea flowers that represent the month of June in Japan.
There is a Japanese legend of an extraordinary strong child ‘Kintaro’, who caught a carp, taller than himself, only with his arms. Carp are familiar to people all over Japan as Koinobori (carp streamer) that is put up in May for their children's healthy growth. In this drawing, Kintaro is showing his extraordinary grip that acts as an external force to drive cellular convergent extension (a process in which a tissue is elongated along a certain axis by cell intercalation in animal development) to elongate the carp's body. The progression status of the convergent extension can be observed by the pattern of the carp's colored scales.
Genome editing technologies have been invented and improved in recent years, and there is a growing expectation that the technologies can be utilized as a tool not only for molecular biological studies, but also for genetic therapies. Among them, CRISPR-Cas9 system that was originally found as a part of the bacterial adaptive immune system gets noticed for its easy introduction. Here, users are only required to design a guide RNA with a 20-nt sequence that is complementary to the target DNA sequence and co-express Cas9 with the RNA in vivo, then Cas9 protein will cleave the target DNA in site-specific manner. This is a portrait of a kabuki actor who plays Cas9 as indicated by the crest “ku” (‘nine’) on kimono he is wearing. The “actor” definitely has a promising future ahead of him.
The fragrance of Japanese plum blossoms is the essence of early spring in Japan. This well-kept plum garden has a unique feature that it is a copy of a landscape of the outer side of animal plasma membrane. The ground represents hydrophilic head domains of the lipid molecules, while the trimmed trunks and branches in the front and the garden stones in the back look like glycosylated membrane proteins. There are a lot of plum trees that look like glycans far off in the distance. A mound on the ground under a basket and a pond in the back express lipid raft (a microdomain that has a different lipid composition) and endocytosis in process, respectively.
An atmospheric ghost light that is called Kitsunebi ('fox light') is described in Japanese folklore. The most famous one is the one in the Oji Inari shrine (Kita-ku, Tokyo). Every New Year's Eve in the old lunar calendar, foxes from all of the Kanto area, all suited up, gather to this shrine, light Kitsunebi under a big tree, and then stand in line. In this drawing, while the line of Kitsunebi in a standard single color is ahead of the rest, the rest of the foxes gathering under the tree are holding up 'new generation Kitsunebi' in various colors and about to join the line. This scene is reminiscent of the green fluorescent protein (GFP; here are three spatial structure models) genetically engineered so as to be available in different emission peaks.
Some dioecious plants determine sex by sex chromosomes (XY or ZW) like mammals or birds. A pair of birds on branches of a pine tree, which is a symbol of a new year in Japan, is a couple of Eclectus parrots (Eclectus roratus) having extreme sexual dimorphism (left: ZZ male; right: ZW female). What they hold in their beaks are flowers of 'Suiba' (common sorrel or Rumex acetosa; left: XY1Y2 male; right: XX female). Suiba is one of the first species among the seed plants whose existence of sex chromosomes was reported (H. Kimura & T. Ono (1923) Bot. Mag. (Tokyo) 37(438), 147-149. DOI: 10.15281/jplantres1887.37.438_147). It is known that Suiba's sex determination system is similar to that of Drosophila and depends on the ratio of X chromosomes to autosomes.
雌雄異体の植物には、哺乳類や鳥類のように性染色体(XYまたはZW)により性決定をしているものがある。日本の新年の象徴のひとつである松の枝に止まっているのは、雌雄で大きく見かけの異なるオオハナインコのつがい(左:ZZ オス; 右:ZW メス)である。彼らが咥えているのはスイバの花である(左:XY1Y2 オス; 右:XX メス)。スイバは種子植物の中では最も早くに性染色体の存在が報告されたもののひとつであり(木原均と小野知夫、1923年)、X染色体と常染色体の比により性決定がなされることが知られている。
Genpaku Sugita (1733-1817) is well-known for his translation of 'Kaitai Shinsho' (New Text on Anatomy, in 1774) compiled based on a book of anatomy written in Dutch. This Japanese first full-blown translation from a Western language contributed to the development of medical science in Japan. The masterpiece has been accomplished while many translated terms such as "shinkei" (= nerve) were coined. Over the past 240 years since then, anatomical methods have dramatically improved. Various optical clearing agents have been invented to clear organs including the brain without difficulty. Now, those agents can make brains transparent without damaging any of the fine structures, and enable researchers to observe neurons deep inside the brain. With our great respect to Genpaku Sugita, let us give him components of the optical clearing agents: urea, detergents and aminoalcohol. Behind him stands a vase of a soapberry tree bearing fruit that used to be raw material for detergents in Edo era.
ニューロンの 雨中も続く 水迷路
An experimental procedure in behavioral neuroscience known as the Morris water maze is a method to assess the animals' ability of spatial learning and memory. In the experiment, test animals are placed in a pool filled with a pool of clouded water where they are supposed to swim, and scientists record the process how the animals find an invisible platform under the water. In this picture, two scientists are recording behavior of mice in an experiment using the Morris water maze. It rains reminding us of dendrites of neurons. One woman puts up an umbrella over the other to prevent the recorder from being wet.
This wooden stand called "Marudai" is used for braiding a Kumihimo, a decorative belt used for tying on a kimono sash. This particular Marudai has the top surface engraved with a part of the codon table in order to translate nucleotide sequences of genes into the pattern of Kumihimo (polypeptide chain). The Marudai and bobbins actually play the role of ribosomes and tRNAs that synthesize proteins by translating mRNA sequences into amino acid sequences. The color of threads had changed depending on amino acids. The pattern of Kumihimo being braided in this drawing was obtained from the sequence of human insulin. Nothing can be as cool as gene sequences, which design the pattern of brilliantly colored Kumihimo.
FoF1 ATPase (also known as ATP synthase) exists in mitochondrial inner membranes and bacterial plasma membranes to synthesize ATP (adenosine triphosphate) by using electrochemical gradient of protons (H+) between inside and outside of the membranes. Fo is integrated in the membranes and thought to have a rotor ring that rotates horizontally. F1 has a dome-like structure comprised of three α and three β subunits that are alternately arranged, which is anchored to the membranes to prevent rotation. Shaft-like γ subunit connects the center of the rotor ring of Fo and F1. As protons flow down the gradient across the membranes through Fo, the rotor ring of Fo and γ rotate, and the spin force of γ against αβ complex is used to synthesize ATP molecules. In this drawing, a waterwheel (Fo) is rotated by the gradient of water (represents proton gradient), and series of three coins (which represent ATP) are generated from the 'apparatus' (αβ of F1), which is made from six boards being attached to the front side of the waterwheel. A man in the back takes a role to transport protons to the outside of membranes to produce proton gradient.
FoF1 ATP合成酵素は、ミトコンドリア内膜や細菌の細胞膜にあまねく存在し、膜内外のプロトン(H+)の電気化学的勾配を利用し、ATPを合成する。Foは膜の中に埋め込まれており、水平に回転する回転子を持っていると考えられている。F1は3つずつのαサブユニットとβサブユニットが交互に並んだ円形ドーム状の構造を持つが、これは膜にアンカーされ回転しない。シャフト状の1つのγサブユニットが、Fo回転子とF1の中心部を接続している。プロトンが勾配に従ってFoを通過する際にFo回転子とγが回転し、αβ複合体に対しγが回転する力でATPが合成される。この画では、水の勾配(プロトンの勾配と見立てている)で水車(Fo)が回転し、水車の手前側にある6枚の板でできた「装置」(F1のαβ)から三つ連なったコイン(ATPを想起させる)が生み出されている様子を描いている。奥の人物は膜の向こう側にプロトンを汲み出し勾配をつくる役割を担っている。
Tsushima Tenno Festival has been held every summer in Tsushima city, Aichi prefecture, Japan, for the past 500 years. One of the highlights of the festival is the evening festival called Yoi-matsuri when five boats decorated hemispherically with a lot of illuminated lanterns come down the river. The distinctive shape of the hemispherical decoration reminds us of partially formed clathrin-coated vesicles (CCV). So this summer, we dare to replace the decorations with the one which looks exactly like spherical CCV after completion. On the front side of the river, we place Y-shaped structures modeled on the LDL (low-density lipoproteins) receptor that mediates CCV's capturing LDL. The reflection of the light from the lanterns which represent CCV sparkles on the water surface of the river, and creates a fantastic summer night view.
愛知県津島市では毎年夏に、500年の伝統を誇る津島天王祭(つしまてんのうまつり)が行われる。クライマックスの一つの宵祭(よいまつり)では多数の提灯を配した半球状の装飾で飾った5艘の船が川を渡る。この半球状の装飾は、まるで形成途中のクラスリン被覆小胞を思わせる特徴的な形状である。そこで今年は思い切って、完成した球状のクラスリン被覆小胞そっくりの装飾を船に乗せてみた。手前の岸にはクラスリン被覆小胞にLDL (low-density lipoproteins)を取り込ませる働きをするLDL受容体を模したY字型の構造物を置いた。夏の夜の川面を、クラスリン被覆小胞を模した提灯飾りが彩り、いかにも幻想的である。
A scene of Yuzen Nagashi (a process of washing kimono silks) at a pontoon bridge over a river. This bridge actually illustrates the way a number of ribosomes (boats) are attached in a row along a single mRNA chain (a bridge) to translate in parallel, and then form a polysome. There is a grove, which is like a poly-A tail, extending from the bridge to the opposite bank of the river. The pieces of fabric form polypeptide chains synthesized by ribosomes with their edges trimmed in the shape of 'M' that is the abbreviation of methionine. Now, the translation processes probably have just terminated at the boats on the far end. The fabric is being folded while it is released from those boats.
広い川にかかる舟橋で友禅流し(着物のための布地を洗う工程)をしている光景。実はこの舟橋は、ひとつのmRNA鎖(橋)に多数のリボソーム(船)が連なって並行して翻訳し、ポリソームを形成している様子をかたどっている。向こう岸には、poly-A tailに似た、橋からつながっている木立が見える。布地はリボソームにより合成されたポリペプチド鎖をかたどっており、布の先端はメチオニンの略号であるMの形状になっている。奥の2艘の船からは、ちょうど翻訳が終結したのだろうか、布地が折りたたまれながら船から遊離しているのが見える。
A parade of “Kappore”, a dance with “nigaigasa” or double layer umbrellas. A decorative piece of cloth modeled after DNA wraps around each umbrella approximately 2 times to link one umbrella to the adjacent umbrellas. This seems to be a replication of the “beads-on-a-string” structure consisting of a DNA strand and nucleosome cores. Those who hold the “nigaigasa” are men representing the four families of core histones. If you look closely, you can find a towel printed “a-ce” on its tail hanging from one of the men's waist. This looks like the acetylation of a tail of one of the core histones. It has been found that the initiation of transcription is related to histone acetylation. If that is the case, this towel may be a sign to start dancing.
Two artisans are heaping up lumber in the form of layers. The process where the lumber is going to be piled up perfectly in six horizontal layers reminds us of the cerebral cortex in corticogenesis. One of the artisans below, on whose back a Chinese character ‘kan’ (‘stem’) can be seen, may play the role of the neural stem cell (radial glia cell), the source of new lumbers (newly created neurons), and extends a rope (radial glia fibers) upward so as to carry the lumber properly to the top layer. The other artisan above is considered to play a role similar to the cells that secrete reelin. The reelin is a protein that regulates correct migration and positioning of neurons.
“Yaedatami” or an eightfold straw mat is a tatami specially prepared for use in religious services. A craftsman folds “tatamiomote” or woven soft rush straws with edging of specially designed Nishijin brocade several times over so as to perfectly align patterns of creases of the folded edgings. We use the design to schematically illustrate the somitogenesis in vertebrates. As the U-shaped presomitic mesoderm moves tailward, periodic waves of gene expression form pairs of somites one after another from the anterior end. “Oshitone” or the top cushion has a mark of an embryo woven into it. If you carefully look at the edging of “oshitone”, you can find it bears the process of segmentation in Drosophila, a species which forms all of its segments simultaneously. Cooperated by Isogaki Tatami (Kyoto, Japan).
FRET (Fluorescence Resonance Energy Transfer), which is a phenomenon of energy transfer between two adjacent fluorescent molecules, has been applied to visualization of interaction between proteins in living cells and so on. To carry out high-efficiency FRET, not only the distance between two molecules must be very close (less than about 10 nm) but the relative orientation of two chromophores must also be optimized. One of the women in this picture carries the other with attention paid on how they are oriented to each other, and successfully lightens the plum blossoms just like FRET.
Mt. Fuji glows with the red tint of the rising sun. A bank of clouds is lying behind the mountain and presenting its characteristic appearance. There are lying clouds in shapes like, from the bottom, rough endoplasmic reticulum, smooth endoplasmic reticulum, and transport vesicles. There is seen above it a bank of clouds well-formed like a Golgi apparatus, and further above it smaller clouds like secretory vesicles. There may also be seen clouds like mitochondria. Wonder if it's just an illusion.
Wishing a prosperous new year, two horses, which carry a red and white twisted rope resembling F-actin, are galloping. This reminds us of pseudopodia that are strongly growing by the power generated from G-actin polymerization.
Following last month's issue, let's imagine what an international academic meeting was like in Edo era. No better place can be found than a Kabuki stage for the oral presentation! The presentation is full house. A presenter (probably the president of this meeting?) stands at the center of the stage, and on his left hand, the chairperson of this session is sitting on the stage. Each paper lantern hanging from the ceiling has a picture of crest imitating model organisms. There is a poster for the 36th Annual Meeting of the Molecular Biology Society of Japan (MBSJ) (3-6 December, 2013, Kobe) behind the presenter.
What if there had been an international academic meeting held in Edo era? It must be like this. This picture illustrates how successfully a poster session was being held. A student-like young man on the far left is performing a presentation on a poster titled “idenshi-kara-saibou-made” (in English, “from Genes to Cells”). Another poster on the right end is the one for the 36th Annual Meeting of the Molecular Biology Society of Japan (MBSJ) (3-6 December, 2013, Kobe). Inside the floor, in the back on the right of the picture, attendees from inside and outside the country are having a discussion over snacks.
もし江戸時代に国際学会を開催したら、こんな感じになったのではないかと想像してみた。この画は、にぎわいを見せるポスターセッションの様子である。左端の若い男性(学生だろうか?)がプレゼンテーションを行っており、そのポスターのタイトルは、ずばり「遺伝子から細胞まで(from Genes to Cells)」である。右方には、第36回日本分子生物学会年会(2013年12月3日~6日,神戸)のポスターが掲示されている。右手奥のフロアー内では、国内外の参加者がスナックをつまみながら議論している。
Protruding from each side of the mountain (represented as cells) like classical cadherin cell adhesion molecules, bridge girders are mutually bound so as to link two summits (cells). This month's cover art represents the mountain surfaces as plasma membranes, the bridge girders and trails as classical cadherins (where each of the bridge girders shows five extracellular cadherin (EC) domains and a transmembrane domain, while the trails show intracellular domains), huts along the trails as catenins, groves of trees as actin filaments, and cliffs as an intercellular space. Sealed with a word “adhesion” at lower right.
Tadataka Inou (1745-1818) was over 50 when he retired from the family business and started to learn Western astronomy. At the age of 55, he led a surveying team to travel throughout Japan islands (40,000 kilometers in total) spending 17 years, and accomplished the first Japanese actual measured map, Dai Nihon Enkai Yochi Zenzu, based on the data collected during the survey. The map created about 200 years ago was so accurate that it later amazed the western surveying teams visiting the country. This month's cover art illustrates Tadataka Inou and his team working together to accomplish a metabolic map. Inspired by Figure 2-35 in The Molecular Biology of the Cell, 5th ed. (Alberts et al. 2008).
伊能忠敬(1745-1818)は元々商人であったが、50歳を過ぎて天文学の勉強を始めた。そして55歳になった伊能忠敬が率いる測量隊は、17年をかけて日本全国を踏破し(計4万km)、そのデータをもとに日本最初の実測地図「大日本沿海輿地(よち)全図」を完成させた。約200年前に作成されたこの地図は、のちに海外からの測量隊を驚嘆させるほど高精度なものであった。本作では代謝マップの作図を成し遂げようとしている伊能忠敬とそのチームの奮闘ぶりを描いている。作画で参考とした文献:Figure 2-35 in The Molecular Biology of the Cell, 5th ed. (Alberts et al. 2008)。
All the traditional Japanese sweets replicate the development of an early embryo from the first cleavage. Each confectionery expresses, from the back, a fertilized egg undergoing the first cleavage (made from “konashi” or kneaded sweet bean paste, coated with “kudzu” starch), a 2-cell-stage embryo (“aoume” or a green plum), an 8-cell-stage embryo (modified from “kiku” or chrysanthemum), a morula (“domyoji” or a sweet pink mochi), and a tailbud (modified from “magatama” or a claw-shaped bead).  So, which one would you like to start with? Confectionery Coordination: Gion Narumiya, Kyoto, Japan.
伝統的な和菓子を用いて、卵割から初期胚の発生までを再現した。奥から順に第一卵割中の受精卵(「くず」でコーティングした「こなし」を用い創作したもの)、2細胞期(「青梅」)、8細胞期(「菊」を改変したもの)、桑実胚(「道明寺」)、尾芽胚(「勾玉」を元に創作したもの)を表現している。いかがだろうか? 和菓子製作協力:京都・祇園ナルミ屋。
相馬野馬追 モデル生物勢、いざ出陣
Soma Nomaoi, Soma horse chasing festival, which used to be a religious ceremony, is now an event held in Soso District (north-eastern part of Fukushima prefecture) every July. In ancient times, Samurais captured wild horses roaming around fields, and dedicated them to Kami. Although wild horses were extinct early in Meiji era, later, the ceremony has been revived as an event in which hundreds of horsemen scramble for holy flags. In this cover art, we let warriors on horsebacks carry flags with arms of model organisms, the strongest tools for our research, to join this event. It is Arabidopsis thaliana (right foreground) what colors the field, the stage for the festival, and streamers of double helix are fluttering in the far background.
相馬野馬追は相双地方(福島県北東部)で毎年7月に行われる祭事であり、かつては野生の馬を捕獲し神に捧げる神事であった。明治時代初期に野生馬は消滅したが、のちに旗を争奪する祭として復活を遂げた。このカバーアートの中だけではあるが、我々が研究する上での最強のツールであるモデル生物の旗印を背負う騎馬武者をこの祭事に参加させてみた。また、この祭りの舞台となる原野をシロイヌナズナ が彩っており(画面右手)、遠くに二重らせんの幟が掲げられている。
kaiA, kaiB and kaiC genes and their products constitute a core circadian oscillator in the cyanobacterium S. elongates (derived from a Japanese term “kai”, which means rotation or cycle number). KaiA stimulates autophosphorylation of KaiC, whereas KaiB promotes autodephosphorylation of KaiC. It is the particular characteristics of system of cyanobacteria that the three purified Kai proteins and ATP can reconstitute a circadian oscillation in a test tube. We overlay a diagram of this feedback loop on an armillary sphere in an Ukiyoe, which was installed at an astronomical observatory in Edo era. The pattern of roof tiles in the foreground depicts a circadian oscillation.
シアノバクテリアS. elongatusの概日周期を作り出す中心となるのがkaiA, kaiB, kaiC遺伝子とその産物である(日本語の「回」に由来)。KaiAはKaiCの自己リン酸化を助け、KaiBはKaiCの自己脱リン酸化を助ける。特に、試験管内で3種類のKaiタンパク質とATPを試験管内で混和させただけでも概日周期の波を再現できるのがシアノバクテリアのシステムの特徴である。このフィードバック・ループのダイヤグラムを、江戸時代の天文台の渾天儀(こんてんぎ)の浮世絵に重ねてみた。手前の屋根の瓦の模様で概日周期の波を表現している。
Speaking of Japanese seasonal tradition in May, you may think of carp streamers flying high over roofs of houses. People fly the carps, wishing for healthy growth of their children. This month, we have painted each scale of a carp streamer, mimicking a mechanism known as lateral inhibition. So, how would you grade the work?
This month's feature is molecular chaperones which support correct protein folding. A child represents a protein (or a polypeptide) trying to fold correctly, while a woman represents a chaperone teaching calligraphy. Pieces of paper scattered on the floor are misfolded polypeptides. In reality, accumulation of misfolded polypeptides will cause various kinds of diseases. Chaperones are essential to life.
A piece inspired by a fusion of traditional Japanese beauty and life sciences. There are a Japanese cosmetic brush and a hand mirror on a black board. An embryo fantastically reflected in the mirror suddenly reminds you of something: the brush is a very micropipette, and the embryo must be a subject of an experiment with it. How cleavage of a fertilized egg occurs is drawn like a family crest on a vanity bag, as if it indicates whom these cosmetic tools belong to. In the embryo, a lacZ marker transgene was driven by a connexin43 (Cx43) promoter in neural crest cells. Courtesy of Osamu Chisaka.
Kites are flying in the early spring in a town with the backdrop of Mt. Fuji. Being steered into the wind and skillfully manipulated via string, the kites look like metaphase chromosomes aligned on the equator of the cell.
Phages have greatly contributed to flourishing of molecular biology, and even today, they remain useful tools for scientists. In this illustration, a “hikeshi” (a firefighter in Edo) is carrying a “matoi” (a standard of his firefighter team as its symbol), which is likened to a phage. Meanwhile lanterns held by firefighters in the back look like bacteria.
当世風龍虎図屏風 其二 粘菌螺旋的龍
The cover art of this month is the second part of the series from the November issue. It describes how cellular slime molds assemble into a dragon. The cellular slime molds normally are individual of an amoeba-like unicellular organism. However, when they starve, tens of thousands of cells aggregate in one place to form a cluster. The cluster hugs the ground like a slug, searches for the right place for making spores, and forms a fluting body (lower part of the ground). When placed in starvation conditions, the cellular slime molds begin to secrete cAMP, which stimulates nearby slime molds to secrete pulses of cAMP. Once they secrete cAMP, they become inactive for a while. As the result, waves of cAMP propagate as they swing in a spiral form (upper part of the sky).
当世風龍虎図屏風 其一 豹紋的虎
The principle mechanism of skin pattern formation in vertebrates can be explained with a mathematical model proposed by Alan Turing (reaction-diffusion system, 1952). In this month's cover art, wild-type and mutant tigers are glaring to the right (at a dragon coming up in December issue). In the front of the wild-type tiger, a rock is illustrated with three peaks representing waves of activator and inhibitor that are observed in the reaction-diffusion system. Furthermore, two bamboos having four internodes represent transmembrane domains in a certain variant of connexin (a subunit of the gap junctions), which is a four-span transmembrande protein and one of the responsible genes for morphological mutation in a vertebrate.
月に雁 繊毛波打つ 秋の宵
Cilia of eukaryotic cells undulate in a well-balanced motion and play a variety of roles essential to life. The cover art for this month's issue presents movements of ciliated cells of respiratory epithelium in an analogy to Susuki glasses, a feature of autumn in Japan. The cilia beat in a given direction is defined by the direction of basal feet (red) that protrude from basal bodies (gray). In the picture, waves to the right are generating a current. There is a goblet cell that secretes mucus, slightly right of the center on the near side of the picture. The moon and geese are also arranged in the picture so as to evoke another feature of autumn in Japan.
The theme of this month is “translation”. The information in DNA is first transcribed into mRNA, which is further converted into a chain of amino acids. This conversion process is called translation and a stop codon marks the end of translation in each mRNA molecule. In the illustration, a fictitious translator on completion of his translation seals the letter with a Chinese character “stop” and amber (which is a nickname for UAG, one of the stop codons). The face of the seal depicts letters of “translation”.
今月の表紙のテーマは「翻訳」である。DNAの情報は一度メッセンジャーRNA (mRNA)へ転写される。さらにmRNAはコドンという3塩基ずつの単位でアミノ酸の配列へと変換され、結果としてアミノ酸が連なったタンパク質が合成される。この変換の過程が翻訳である。翻訳を終える目印もコドンであり、終止コドンと呼ばれる。この画像では、架空の『翻訳家』が「止」(stop)の文字と琥珀(終止コドンの1つであるUAGの愛称が琥珀(amber))の位置で、自らが為すべきことを終えたと認め押印した様子を描画している。落款の印面には「翻訳」の文字がデザインされている。
Coloring the summer night sky of Japan, this firework has a theme of the half spindle of animal mitotic cells. A centrosome containing a pair of centrioles is located in the center, and astral microtubules extend to the up to the left, while polar microtubules extend to the opposite direction. Japanese technology currently produces cutting-edge fireworks, and it should not be difficult to produce such fireworks in reality.
This cover depicts The Bridge in the NGS Rain where people are being exposed to torrents of genomic information, that is, fruits of next generation sequencing. The man standing in the middle of the bridge is pictured deep in thought, considering how to utilize this sequence data set of the ALDH2 gene. Another man, shown to the right with a sake bottle, is eager to know whether he is genetically predisposed with a high tolerance for alcohol or not.
The Epigenetic landscape, which was firstly depicted by C. H. Waddington in 1950's, has often been used to illustrate cellular differentiation in the process of biological development. Presented on our cover is a timeless representation of the landscape where we see worshippers of Mt. Fuji are being reprogrammed on the slope. Some are climbing high to acquire pluripotency, while others are traversing the foot, and being directly reprogrammed.
エピジェネティック・ランドスケープは、C. H. Waddingtonにより1950年代に提唱された、発生における分化に対する比喩である。我々も富士山におけるエピジェネティック・ランドスケープの図を伝統的なタッチで描いてみた。山頂に向って登る参詣者は、未分化状態に戻って多能性を獲得する細胞を連想させる。また、裾野を横へ行く参詣者は、未分化状態に戻らないダイレクト・リプログラミングのさなかの細胞を表現している。
Regulated cell adhesion and cellular motility underlies dynamic cell rearrangement, which is essential for morphogenesis of various organs. We expressed this process using a picture of a stone levee along a canal.
Branched waterfalls are reminiscent of the cell lineage of the nematode Caenorhabditis elegans from a zygote to a newly hatched larva.
枝分かれする滝の情景で、線虫Caenorhabditis elegansの受精卵から幼虫に至る細胞系譜が表現されている。
A woman with a hanagasa (a traditional hat decorated with ornamental flowers) is performing a Japanese classical dance. The ornaments on the hat actually are gap junctions, and some of them are opened and allowing small molecules to pass through.
A fisherman on a cliff is pulling nets as if synchronizing circadian rhythms with the help of light shining down from the sky.
Set against the backdrop of Mt. Fuji, a skilled craftsman is fixing a broken wooden barrel by excising damaged bases and then introducing new ones. We liken this to the process of DNA repair thereby recovering hydrogen bonds between each pair of bases.
Since nerves of higher animals have myelin sheaths, an action potential is propagated rapidly (phenomenon called a saltatory conduction) like the swift wind. The wind god is likened to an action potential that conduct bounces to be propagated.
The Thunder God is generating the action potential, which is not lightning, in a neural network. The illustration is paired with that of December Issue.
Harvest time. Toreutics on a guard of a Japanese sword has a design of ears of rice and a butterfly landing on them. No, they actually are DNA winding around nucleosome.
A huge and prestigious shrine that looks like Izumo-Taisha shrine at a glance. White zigzag paper streamers like a leucine zipper-type transcription factor are tucked in a giant sacred rope (dextrorotatory double-helix). The paper streamers probably bind to specific sequences of dsDNA expressed as a sacred rope.
Being inspired by the similarity in the appearance, KOKESHIs are portrayed as metaphase chromosomes, which pair KOKESHIs. It deserves attention that each pair of KOKESHI is designed the same and tied with a thread at their necks (kinetochores) each other.
Importance of bidirectional transportation between TOHOKU and surrounding districts. The “Kaido” expressed as a stone-pave street is designed to look like microtubules. Carts going left and pulled by bulls and messengers going right are expressions of motor proteins with cargos in eukaryote cells.
A scene from a Chinese-inspired vigorous dragon dance in a festival in Kyushu, Japan. The dragon is likened to a messenger RNA (mRNA), expressing transcription with DNA template.
九州地方に伝わる中国風の躍動感あふれる龍踊りの祭りの風景より。龍をメッセンジャーRNA(mRNA)と見立て、DNAを鋳型 にして転写されている状況を描写している。
A beautiful woman of the Heian period is pulled by a dog and peeks out from behind a bamboo blind, which is likened to experimental data of DNase I footprint. Data:Akita et al. Genes to Cells 15(5) pp537-552 (2010).
DNase Iによるフットプリントの実験データを簾に見立て、平安時代の美女が犬に引かれ簾から顔をのぞかせている画を描いた。データは、Akita et al. Genes to Cells 15(5) pp537-552 (2010) より。
Grand Sumo showing a YOKOZUNA or grand champion and “hands” of supporters are pulling the horizontal rope of the kesho-mawashi. They want to tie the kesho-mawashi, but simultaneously, they are making a new horizontal rope from an older vertical rope, using Holliday junction-like structure.
A designed family emblem of cell division blends well with traditional Japanese patterns of auspicious omens motifs as pine and bamboo, on the artwork of tiered food boxes in silver lacquer.
Two sets of segregated chromatids during telophase are depicted as cats cuddled up comfortably beside spindle poles.
Overlooking Mount Fuji in the distance through the arching wooden bridge, reflected on the water surface, forming complete circles to resemble a pair of spectacles or a ‘double helix.’