Genome editing technologies have been invented and improved in recent years, and there is a growing expectation that the technologies can be utilized as a tool not only for molecular biological studies, but also for genetic therapies. Among them, CRISPR-Cas9 system that was originally found as a part of the bacterial adaptive immune system gets noticed for its easy introduction. Here, users are only required to design a guide RNA with a 20-nt sequence that is complementary to the target DNA sequence and co-express Cas9 with the RNA in vivo, then Cas9 protein will cleave the target DNA in site-specific manner. This is a portrait of a kabuki actor who plays Cas9 as indicated by the crest “ku” (‘nine’) on kimono he is wearing. The “actor” definitely has a promising future ahead of him.
The fragrance of Japanese plum blossoms is the essence of early spring in Japan. This well-kept plum garden has a unique feature that it is a copy of a landscape of the outer side of animal plasma membrane. The ground represents hydrophilic head domains of the lipid molecules, while the trimmed trunks and branches in the front and the garden stones in the back look like glycosylated membrane proteins. There are a lot of plum trees that look like glycans far off in the distance. A mound on the ground under a basket and a pond in the back express lipid raft (a microdomain that has a different lipid composition) and endocytosis in process, respectively.