Vol. 21, No. 2(February 2016)

In the most popular type of next-generation DNA sequencer, DNA fragments are PCR-amplified in the slide's channel coated by a lawn of PCR primers to form myriads of clone DNA clusters. After that, the DNA sequence of each cluster is determined by taking photographs of fluorescent signals on the slide with the image sensor again and again after each single-nucleotide extension step of a novel dye-terminator method. Thus, the reaction proceeding in the next-generation sequencer is massively parallel sequencing. In this drawing, we schematically expressed this mechanism with a flying hawk (image sensor) viewing clusters of pine trees (DNA) on the channel-shaped reclaimed land divided by embankments.

---

Genes to Cells © 2016 the Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

Volume 21, Issue 2, 2016.

Original Articles

Pages: 122-135 遺伝学的相補解析によって示された、エピジェネティック制御におけるヒストンバリアントH2A.Z N末端テールの多様な機能 Genetic complementation analysis showed distinct contributions of the N-terminal tail of H2A.Z to epigenetic regulations 日下部　将之、奥　裕之、松田　涼、堀　哲也、武藤　哲彦、五十嵐　和彦、深川　竜郎、原田　昌彦 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12327

Pages: 136-145 長い逆向き反復配列ではDNA複製フォークの進行がリーディング・ラギング鎖上に形成されるヘアピン構造により阻害される Long inverted repeat transiently stalls DNA replication by forming hairpin structures on both leading and lagging strands Lai Pey Jiun、Lim Chew Theng、Le Phuong Hang、片山　勉、David R. F. Leach、古郡　麻子、真木　寿治 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12326

Pages: 146-162 Eomesoderminはマウス胸腺腫のIFN-γ発現を促進し、Ifng遺伝子座の複数の保存された非コード配列に結合する Eomesodermin promotes interferon-γ expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines 福岡　菜月、原田　美鈴、西田　愛、伊藤　ゆう子、塩田　秀樹、片岡　孝夫 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12328

Pages: 163-184 SSB binds to the RecG and PriA helicases in vivo in the absence of DNA Cong Yu, Hui Yin Tan, Meerim Choi, Adam J. Stanenas, Alicia K. Byrd, Kevin Raney, Christopher S. Cohan and Piero R. Bianco https://onlinelibrary.wiley.com/doi/10.1111/gtc.12334

Pages: 185-199 MEK-ERK-phospho-STAT3 (Ser727)経路により誘導されるMcl-1L高発現は、メラノサイトとメラノーマ細胞においてUVBによるアポトーシスを抑制する High expression of Mcl-1L via the MEK-ERK-phospho-STAT3 (Ser727) pathway protects melanocytes and melanoma from UVB-induced apoptosis 福本　毅、岩崎　哲史、岡田　太郎、橋本　貴範、文　有彬、坂口　正展、深見　泰夫、錦織　千佳子、岡　昌宏 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12330

Pages: 200-211 大きな非線維型アミロイドβ凝集体形成の免疫組織染色による観察 Immunohistochemical detection of the delayed formation of nonfibrillar large amyloid-β aggregates 落石　知世、板倉　暁、劉　磊、赤津　裕康、神野　英毅、西村　正樹、吉宗　一晃 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12332