Vol. 20, No. 4(April 2015)

Genome editing technologies have been invented and improved in recent years, and there is a growing expectation that the technologies can be utilized as a tool not only for molecular biological studies, but also for genetic therapies. Among them, CRISPR-Cas9 system that was originally found as a part of the bacterial adaptive immune system gets noticed for its easy introduction. Here, users are only required to design a guide RNA with a 20-nt sequence that is complementary to the target DNA sequence and co-express Cas9 with the RNA in vivo, then Cas9 protein will cleave the target DNA in site-specific manner. This is a portrait of a kabuki actor who plays Cas9 as indicated by the crest “ku” (‘nine’) on kimono he is wearing. The “actor” definitely has a promising future ahead of him.

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Genes to Cells © 2015 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd

Volume 20, Issue 4, 2015.

Original Articles

Pages: 257-266 マウス網膜由来の培養細胞において、HERMES/RBPMSはNonO, PSF, G3BP1と神経細胞質RNP顆粒を形成する Interaction and colocalization of HERMES/RBPMS with NonO, PSF, and G3BP1 in neuronal cytoplasmic RNP granules in mouse retinal line cells 古川　真理、坂本　博、井上　邦夫 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12224

Pages: 267-280 C18orf42はプロテインキナーゼAに結合する新規タンパク質をコードしている Hypothetical gene C18orf42 encodes a novel protein kinase A-binding protein 福田　牧葉、相澤　康則 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12217

Pages: 281-291 エクスポーティン５は脊椎動物のSRP RNAの核外輸送を司る Exportin-5 mediates nuclear export of SRP RNA in vertebrates 竹岩　俊彦、谷口　一郎、大野　睦人 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12218

Pages: 292-309 ケミカルゲノミクスの手法を用いた、分裂酵母のラパマイシン感受性遺伝子群の網羅的同定 Chemical genomics approach to identify genes associated with sensitivity to rapamycin in the fission yeast Schizosaccharomyces pombe 土井　章、藤本　明由美、佐藤　駿、宇野　貴哉、神田　勇輝、浅海　慶太、田中　夕莉子、喜多　綾子、佐藤　亮介、杉浦　麗子 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12223

Pages: 310-323 ゲラニルゲラニルトランスフェラーゼCgw2はRho4/Rho5を介して分裂酵母のPmk1 MAPK経路を制御する Geranylgeranyltransferase Cwg2-Rho4/Rho5 module is implicated in the Pmk1 MAP kinase-mediated cell wall integrity pathway in fission yeast 土井　章、喜多　綾子、神田　勇輝、宇野　貴哉、浅海　慶太、佐藤　亮介、中野　賢太郎、杉浦　麗子 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12222

Pages: 324-339 cnrip1はアフリカツメガエルの目と神経の発生の制御因子である cnrip1 is a regulator of eye and neural development in Xenopus laevis 鄭　小娜、鈴木　俊康、高橋　知佳、西田　栄介、日下部　杜央 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12225

Pages: 340-357 転写因子forkhead box O1の核膜移行に伴うヒストンの化学修飾変化によりPCK1遺伝子の転写が制御されている Translocation of forkhead box O1 to the nuclear periphery induces histone modifications that regulates transcriptional repression of PCK1 in HepG2 cells 荒井　珠貴、加納　ふみ、村田　昌之 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12226

Brief Report

Pages: 358-365 脂肪代謝におけるショウジョウバエMESR4遺伝子の新規機能 Identification of a novel role for Drosophila MESR4 in lipid metabolism 津田（櫻井）　香代子、成　耆鉉、堀内　純二郎、相垣　敏郎、津田　学 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12221