Vol. 26, No. 5(May 2021)

This “Daruma Otoshi” game imitates the VDJ recombination of the T cell receptor β (TCRβ) locus. In the first step, the DNA sequence between one D and one J is removed, and in the second step, the DNA sequence between one V and the said D is removed. The series of reactions is catalyzed by RAG1/RAG2. The current situation is that the first stage has been successfully completed and we are in the middle of the second step, that is, the DNA sequence between V_β3-1 and D_β1 is being knocked out with a hammer marked with RAG1 and RAG2. Since there are two alleles, there are two chances, but if both fail, the cell will undergo apoptosis.

---

Genes to Cells © 2021 the Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

Volume 26, Issue 5, 2021.

Original Articles

Pages: 269-281 ショウジョウバエ胚発生期において、polished rice遺伝子はエクジソン依存的に器官形成を制御している polished rice mediates ecdysone-dependent control of Drosophila embryonic organogenesis 平　雄樹、和田　宝生、林　茂生、影山　裕二 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12841

Pages: 282-297 Sonic hedgehog signals hinder the transcriptional network necessary for pancreatic endoderm formation from human embryonic stem cells Niloufer P. Dumasia, Aparna Khanna and Prasad Pethe https://onlinelibrary.wiley.com/doi/10.1111/gtc.12839

Pages: 298-312 ヒト生細胞におけるRNAポリメラーゼII凝集体の形成とカハルボディ、ヒストン ローカスボディとの相互作用 RNA polymerase II condensate formation and association with Cajal and histone locus bodies in living human cells 今田　貴士、志見　剛、海保　愛、佐伯　泰、木村　宏 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12840

Pages: 313-327 インターフェロンα誘導性樹状細胞におけるGZMB/PRF1/PI-9 の発現の高いCD56^dimサブポピュレーションの同定 Identification of CD56^dim subpopulation marked with high expression of GZMB/PRF1/PI-9 in CD56⁺ interferon-α-induced dendritic cells 渡部　明日香、硎　美紗、小屋　照継、谷口　真、坂本　卓弥、岩淵　邦芳、加藤　友久、下平　滋隆 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12844

Brief Reports

Pages: 328-335 ヒトSIRT2、SIRT3脱アセチル化酵素は、DNA相同組換え修復に関わる Human SIRT2 and SIRT3 deacetylases function in DNA homologous recombinational repair 安田　武嗣、滝澤　和也、宇井　彩子、濱　三知夫、香川　亘、菅澤　薫、田嶋　克史 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12842

Pages: 336-343 ポリビニルアルコールはアポトーシスの抑制と細胞増殖の促進を介して、ヒトがん細胞の三次元培養を支持する Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation 沖田　結花里、鄭　齢、川西　邦夫、三好　浩稔、栁原　五吉、加藤　光保 https://onlinelibrary.wiley.com/doi/10.1111/gtc.12843